We narrowed to 13,284 results for: sequence
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Plasmid#209872PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1DepositorInsertddRFP A
TagsTargeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Matrix-roGFP
Plasmid#49437PurposeExpresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cellsDepositorInsertMatrix-roGFP2
UseAdenoviralTagsCytochrome c oxidase subunit IV mitochondrial tar…ExpressionMammalianMutationC48S, Q80R, S147C, and Q204C in Clontech's G…PromoterCMVAvailable SinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV BiP-Myc-KDEL-wt
Plasmid#27164PurposeMammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequenceDepositorAvailable SinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
Mito-GA
Plasmid#209865PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVSDepositorInsertddGFP A
TagsTargeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEC3115 (pSpy0K6-P23_mKate2~*ssrA)
Plasmid#218509Purposeintegrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombinationDepositorInsertmKate2
TagsssrA degradation tagExpressionBacterialMutationDeletion of Plac_mrfp cassette, introduction of a…PromoterP23Available SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Cas9-T2A-GFP
Plasmid#63155PurposeMiddle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.DepositorInsertCas9-T2A-GFP
UseCRISPR; Tol2 middle entry vector for gatewayAvailable SinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Fis1
Plasmid#182580PurposeExpresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequenceDepositorInsertmCherry-Fis1
TagsFis1ExpressionMammalianAvailable SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMVTNT-T7-KPNA1
Plasmid#26677DepositorInsertKaryopherin Alpha 1 (KPNA1 Human)
TagsT7ExpressionMammalianMutationKPNA1 sequence contains a reported SNP at S73NAvailable SinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-msfGFP
Plasmid#194913PurposeTetracycline inducible expression of msfGFP in StaphylococciDepositorInsertShine Dalgarno Sequence followed by monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…ExpressionBacterialAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro
Plasmid#180273PurposeLentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassetteDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only