We narrowed to 157,076 results for: Des

1. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

   Plasmid

   #216283

   Purpose

   Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SpyCatcher003-TEVs-SpyTag003DA
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDest-311 (attR4-attR3)

   Plasmid

   #161896

   Purpose

   Multisite Gateway (attR4-attR3) vector for mammalian protein expression

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. LB601: pMVP/PB/eGFP-DEST

   Plasmid

   #121870

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ eGFP selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFRT/TO/FLAG/HA-DEST DICER

   Plasmid

   #19881

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   Dicer 1, ribonuclease type III (DICER1 Human)
   

   Tags

   FLAG/HA

   Expression

   Mammalian

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST mpeg1:tomato-CAAX

   Plasmid

   #135215

   Purpose

   Red tagged macrophages

   Depositor

   David Tobin

   Article

   Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

   Insert

   mpeg1 and tdTomato
   

   Use

   Zebrafish expression

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-FRT/T0-GFP-PALB2

   Plasmid

   #71113

   Purpose

   mammalian expession of GFP-PALB2

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   PALB2 (PALB2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV/TO

   Available Since

   Dec. 9, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pDEST-FRT/TO-eGFP-REV7

   Plasmid

   #114128

   Purpose

   Expresses N-terminally tagged eGFP-REV7 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   REV7 (MAD2L2 Human)
   

   Use

   Genomic integration, tetracycline inducible

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV/TetO2

   Available Since

   Aug. 15, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pcDNA3.2-V5-DEST-hSFRP1-his

   Plasmid

   #189540

   Purpose

   Protein expression of SFRP1 in mammalian cells.

   Depositor

   Haian Fu

   Insert

   secreted frizzled related protein 1 (SFRP1 Human)
   

   Tags

   his

   Expression

   Mammalian

   Available Since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST40-2XFL-Src-Y530F

   Plasmid

   #140319

   Purpose

   Expresses Flag-tagged human Src-Y530F in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   Y530F

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-FRT/T0-Flag-BRCA1

    Plasmid

    #71117

    Purpose

    mammalian expression of Flag-BRCA1 siRNA resistant construct

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV/TO

    Available Since

    Nov. 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pDESTN-MTp-p53-Venus

    Plasmid

    #53736

    Purpose

    Expresses Venus tagged p53 under the metallothionein promoter

    Depositor

    Galit Lahav

    Article

    Batchelor et al Mol Cell. 2008 May 9;30(3):277-89. doi:

    Insert

    p53 (TP53 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    metallothionein promoter

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-Hyg-Dest-mCherry-PAR-6B

    Plasmid

    #194908

    Purpose

    To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

    Depositor

    Clare Buckley

    Article

    Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

    Insert

    PAR-6B (Pard6b Mouse)
    

    Use

    Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    39 C to G, silent mutation

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

    Plasmid

    #229828

    Purpose

    A Piggybac-compatible, cre-dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti CMV/TO DEST (775-1)

    Plasmid

    #17432

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. LC201: pMVP/PB/Puro-DEST

    Plasmid

    #121874

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti X2 Puro DEST (w16-1)

    Plasmid

    #17296

    Purpose

    3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Puro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi; Destination vector

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDest-676 (attR4-attR3)

    Plasmid

    #161888

    Purpose

    Multisite Gateway (attR4-attR3) vector for lentivirus production

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDestTol2CG2-mnx1:ReaChR-citrine

    Plasmid

    #169046

    Purpose

    Destination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

    Depositor

    Paola Arlotta

    Article

    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

    Insert

    ReaChR-Citrine
    

    Tags

    Citrine

    Expression

    Mammalian

    Promoter

    3xMnx1

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Omega Destination-CMV::ELuc:bGH

    Plasmid

    #124528

    Purpose

    Omega destination vector, that has a CMV:ELUC:bgH in the backbone

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Type

    Empty backbone

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-T7-TALEN(Sangamo)-Destination

    Plasmid

    #37184

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Tags

    FokI nuclease

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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