We narrowed to 2,543 results for: LRE

Showing: 1661 - 1680 of 2543 results

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pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP

Plasmid

#166150

Purpose

Expression of N. furzeri DDX5ΔPrD(1-535) mutant in E. coli

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5ΔPrD(1-535)

Tags

His10-Smt3 and SNAP

Expression

Bacterial

Mutation

ΔPrD(1-535)

Promoter

T7

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-Smt3-DDX5ΔIDR(kf)-SNAP

Plasmid

#166151

Purpose

Expression of N. furzeri DDX5ΔIDR(1-483) mutant in E. coli

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5ΔIDR(1-483)

Tags

His10-Smt3 and SNAP

Expression

Bacterial

Mutation

ΔIDR(1-483)

Promoter

T7

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-mKO2-Parkin

Plasmid

#213544

Purpose

Transient mammalian expresssion of Parkin, N-terminally tagged with mKO2, M1 of Parkin intact

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Tags

mKO2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Parkin(E16A)

Plasmid

#213546

Purpose

Transient mammalian expresssion of E16A Parkin

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Expression

Mammalian

Mutation

E16A mutation in Parkin

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGMC00033 (aka pMC0224)

Plasmid

#210750

Purpose

Guide RNA targeting SERPINB3

Depositor

Raj Chari

Article

Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

Insert

SERBPINB3 guide RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_I105N__C192A

Plasmid

#201176

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, I105N, C192A

Promoter

TRE3G

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_C192A

Plasmid

#201172

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, C192A

Promoter

TRE3G

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_I105N_C39A

Plasmid

#201175

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, I105N, C39A

Promoter

TRE3G

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_C39A

Plasmid

#201171

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, C39A

Promoter

TRE3G

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcmv3-IRG1-H277Y-HA

Plasmid

#198185

Purpose

Mammalian expression of HA-tagged human ACOD1 (IRG1) H277Y

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

ACOD1 (ACOD1 Human)

Tags

HA

Expression

Mammalian

Mutation

The histidine in IRG1 at position 277 mutates to …

Promoter

CMV

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-MYO10ΔF3

Plasmid

#194857

Purpose

Express EGFP-MYO10 with the F3 FERM domain lobes deleted (1-1951) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

MYO10 ΔF3 (MYO10 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Truncated MYO10 construct. MYO10 amino acids 1-1…

Promoter

CMV

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRps9-WT-IRES-GFP-HygR

Plasmid

#190271

Purpose

Express RPS9 WT mouse gene transcriptionally fused with eGFP. eGFP is translated from IRES.

Depositor

Erik Böttger

Article

Brilkova et al Cell Rep. 2022 Sep 27;40(13):111433. doi

Insert

ribosomal protein RPS9

Tags

IRES-eGFP

Expression

Mammalian

Promoter

CAGGS-CMV

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pRps9-D95N-IRES-GFP-HygR

Plasmid

#190272

Purpose

Express RPS9 D95N-mutant mouse gene transcriptionally fused with eGFP. eGFP is translated from IRES.

Depositor

Erik Böttger

Article

Brilkova et al Cell Rep. 2022 Sep 27;40(13):111433. doi

Insert

ribosomal protein RPS9

Tags

IRES-eGFP

Expression

Mammalian

Mutation

D95N

Promoter

CAGGS-CMV

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-RG-Linker-eGFP

Plasmid

#176071

Purpose

EGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

RG

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO-3XFLAG Halo R99A nsp1

Plasmid

#175422

Purpose

Expresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 R99A nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-MCS-LexA-VP16-MiniWhite

Plasmid

#165900

Purpose

Blasticidin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable Empty Enhancer Driver

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEST42 -SSRP1

Plasmid

#170439

Purpose

Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

ssrp1 (Ssrp Fly)

Tags

6xHis and V5

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO ACD11

Plasmid

#170738

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Cell Rep. 2014 Jan 30;6(2):388-99. doi:

Insert

acd11 (ACD11 Mustard Weed)

Tags

SUMO

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA2 R2896C

Plasmid

#139511

Purpose

Plasmid expressing a sgRNA to introduce BRCA2 R2896C using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA2 E2896C using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-Y530EEI

Plasmid

#140322

Purpose

Expresses HA-tagged human Src-Y530EEI in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

Y530EEI

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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