We narrowed to 1,686 results for: SUA

1. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

   Plasmid

   #99277

   Purpose

   Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance

   Depositor

   Jordan Miller

   Article

   Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

   Inserts

   H2B-mOrange2-IRES-EGFP
   Puro
   

   Use

   Lentiviral

   Tags

   EGFP, H2B, IRES, PGK, and mOrange2

   Expression

   Mutation

   Promoter

   EF1 alpha-human and PGK-mouse

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSA2

   Plasmid

   #52262

   Purpose

   Bacterial expression of human 6His-tagged SUMO2 and SUMO-E1

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Inserts

   SUMO-2 (SUMO2 Human)
   SAE-2 (UBA2 Human)
   SAE-1 (SAE1 Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   C-terminal gly-gly

   Promoter

   T7 and rbs

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

   Plasmid

   #99278

   Purpose

   Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance

   Depositor

   Jordan Miller

   Article

   Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

   Inserts

   H2B-mVenus-IRES-mCherry
   Puro
   

   Use

   Lentiviral

   Tags

   H2B, IRES, PGK, mCherry, and mVenus

   Expression

   Mutation

   Promoter

   EF1 alpha-human and PGK-mouse

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cherry-HA-KCC2

   Plasmid

   #104077

   Purpose

   To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

   Depositor

   Igor Medyna

   Article

   Kahle et al EMBO Rep. 2014 Jul;15(7):766-74. doi: 10

   Insert

   KCC2 (b isoform) (Slc12a5 Rat)
   

   Use

   Tags

   mCherry-HA tag

   Expression

   Mammalian

   Mutation

   EcoR1 site in rat KCC2 was removed by silent muta…

   Promoter

   UbC

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSA3

   Plasmid

   #52263

   Purpose

   Bacterial expression of human 6His-tagged SUMO3 and SUMO-E1

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Inserts

   SUMO-3 (SUMO3 Human)
   SAE-2 (UBA2 Human)
   SAE-1 (SAE1 Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   C-terminal gly-gly

   Promoter

   T7 and rbs

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIBN-LacI-tagRFP-T

   Plasmid

   #103810

   Purpose

   'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-LacI-tagRFP-T (CIB1, lacI Mustard Weed, E. coli)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   LacI: C19T (silent, L7L), T264C (silent, A88A), C…

   Promoter

   CMV

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

   Plasmid

   #190181

   Purpose

   Fluorescent reporter vector to visualize all of cell cycle phases.

   Depositor

   Toshiro Sato

   Article

   Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

   Inserts

   iRFP
   hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
   TagBFP2
   hGeminin(1/110)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-EF1a-mCherry-EGFP-LC3B

   Plasmid

   #170446

   Purpose

   Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

   Depositor

   Sang-Hun Lee

   Article

   Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

   Insert

   mCherry-GFP-LC3 (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMIH-TFAM-GFP

   Plasmid

   #113704

   Purpose

   Fluorescent fusion protein used to visualise mitochondrial nucleoids, with hygromycin selection

   Depositor

   Benjamin Kile

   Article

   McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

   Insert

   TFAM (Tfam Mouse)
   

   Use

   Retroviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMIH-TOMM20-Halo

    Plasmid

    #111626

    Purpose

    Fusion protein used to visualise mitochondria upon the addition of Halo specific dye, has hygromycin selection

    Depositor

    Benjamin Kile

    Article

    McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

    Insert

    TOMM20 (Tomm20 Mouse)
    

    Use

    Retroviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Fucci(CA) hCdt1-iRFP hGeminin-iRFP

    Plasmid

    #190182

    Purpose

    Fluorescent reporter vector for visualization of G0 and other cell cycle phases.

    Depositor

    Toshiro Sato

    Article

    Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

    Inserts

    iRFP
    hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
    iRFP
    hGeminin(1/110)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMIH-mRuby2-BAX

    Plasmid

    #111628

    Purpose

    Fluorescent fusion protein used to visualise mouse BAX, with hygromycin selection

    Depositor

    Benjamin Kile

    Article

    McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

    Insert

    Bax (Bax Mouse)
    

    Use

    Retroviral

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMIH-mRuby2-BAK

    Plasmid

    #111627

    Purpose

    Fluorescent fusion protein used to visualise mouse BAK, with hygromycin selection

    Depositor

    Benjamin Kile

    Article

    McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

    Insert

    BAK (Bak1 Mouse)
    

    Use

    Retroviral

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCIBN-hTRF1-tagRFP-T

    Plasmid

    #103811

    Purpose

    BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-hTRF1-tagRFP-T (CIB1, TERF1 Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. FUW-hSNCA(D2R)-GFP

    Plasmid

    #215490

    Purpose

    Lentiviral expression and easy visualization of mutated alpha-synuclein (2nd amino acid aspartate mutated to arginine) in mammalian cells

    Depositor

    Ophir Shalem

    Article

    Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D (second aa) mutated to R

    Promoter

    Ubc

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUW-hSNCA(D2E)-GFP

    Plasmid

    #215491

    Purpose

    Lentiviral expression and easy visualization of mutated alpha-synuclein (2nd amino acid aspartate mutated to glutamate) in mammalian cells

    Depositor

    Ophir Shalem

    Article

    Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D (second aa) mutated to E

    Promoter

    Ubc

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-Sport6-CD63-pHluorin

    Plasmid

    #130901

    Purpose

    Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHluorin (CD63 Aequorea victoria, Human)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Gln-36 and Leu-37 in ex…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-Sport6-CD63-pHuji

    Plasmid

    #130902

    Purpose

    Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHuji (CD63 Human, synthetic)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Gln-36 and Leu-37 in extra…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-Sport6-CD81-pHuji

    Plasmid

    #130904

    Purpose

    Expression of CD81-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD81-pHuji (CD81 Human, Synthetic)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Leu-49 and Gly-50 in extra…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-Sport6-CD9-pHluorin

    Plasmid

    #130905

    Purpose

    Expression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD9-pHluorin (CD9 Aequorea victoria, Human)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    phluorin inserted between Asn-50 and Asn-51 in ex…

    Promoter

    CMV

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge