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We narrowed to 4,850 results for: U6...

Showing: 1661 - 1680 of 4850 results
  1. pMLS256

    Plasmid
    #73715
    Purpose
    SapTrap destination vector for building combined sgRNA expression + repair template vectors
    Depositor
    Insert
    pU6::sgRNA
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    SapI insertion site
    Promoter
    C. elegans U6 snRNA pol III promoter
    Available since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid
    #118157
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS
    Depositor
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pLKO.puro_shHuR 3UTR

    Plasmid
    #110414
    Purpose
    TRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, puromycin selection
    Insert
    ELAVL1 (HuR)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (RNA PolIII)
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA

    Plasmid
    #191528
    Purpose
    Vector for tandem expression of SLBP 3'UTR G1 sgRNA in combination with ATP1A1 G3 sgRNA from two independent U6 promoters to facilitate SLBP endogenous tagging by marker free coselection using ouabain
    Depositor
    Insert
    SLBP 3'UTR G1 sgRNA + ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pLKO.1 LARP7-FLAG m6A mut 7SK

    Plasmid
    #200734
    Purpose
    Lentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SK
    Depositor
    Insert
    LARP7 and 7SK (LARP7 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    7SK m6A mutations: A172G, A220T, A228G, A281G, an…
    Promoter
    U6
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid
    #118159
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS
    Depositor
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 1661 - 1680 of 4850 results