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U371-GFP ILtoEK

Plasmid

#24472

Depositor

Ron Vale

Article

Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

Insert

Unc104 (unc-104 Nematode)

Tags

GFP

Expression

Bacterial

Mutation

Contains only the kinesin's motor head fused…

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
pLJ54 (Pmyo-2_GCaMP8f_SL2_mKate2_let-858_3'UTR)

Plasmid

#239864

Purpose

expression of GCaMP8f in C. elegans under myo2-promoter control

Depositor

Monika Scholz

Article

Liu et al Genetics. 2024 Oct 7;228(2):iyae125. doi

Inserts

GCaMP8f
mKate2

Tags

6xHis

Expression

Worm

Mutation

codon optimized with introns for C. elegans

Promoter

myo-2

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pREA3

Plasmid

#245832

Purpose

Express rac-2(G12V) in all neurons.

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

rac-2(G12V) (rac-2 Nematode)

Expression

Worm

Mutation

G12V

Promoter

rgef-1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pREA12

Plasmid

#245770

Purpose

Allows for mCherry::RBD::T2A::rac-2::mGFP entry into Gateway Vectors

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

mCherry::RBD::T2A::rac-2::mGFP

Use

Unspecified

Tags

mCherry and mGFP

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pREA16

Plasmid

#245771

Purpose

Expresses mCherry::RBD::T2A::rac-2::mGFP in cholinergic motor neurons

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

mCherry::RBD::T2A::rac-2::mGFP

Tags

mCherry and mGFP

Expression

Worm

Promoter

unc-17b

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSJC321

Plasmid

#245777

Purpose

Expresses rac-2(G12V)::mGFP in cholinergic motor neurons

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

rac-2(G12V)::mGFP

Tags

mGFP

Expression

Worm

Mutation

G12V

Promoter

unc-17b

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSJC319

Plasmid

#245775

Purpose

Expresses rap-1(G12V)::mGFP in cholinergic motor neurons

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

rap-1(G12V)::mGFP

Tags

mGFP

Expression

Worm

Mutation

G12V

Promoter

unc-17b

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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IDH1_WT

Plasmid

#224142

Purpose

IPTG-inducible expression of C. elegans protein IDH-1 with a chitin tag

Depositor

Katherine Walstrom

Article

Bouchard et al Int J Mol Sci. 2025 Aug 25;26(17):8238.

Insert

IDH-1 (F59B8.2 Nematode)

Tags

intein-CBD

Expression

Bacterial

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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IDH1_G98N

Plasmid

#224143

Purpose

IPTG-inducible expression of C. elegans protein IDH-1(G98N) with a chitin tag

Depositor

Katherine Walstrom

Article

Bouchard et al Int J Mol Sci. 2025 Aug 25;26(17):8238.

Insert

IDH-1(G98N)

Tags

intein-CBD

Expression

Bacterial

Mutation

G98N

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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IDH1_R133H

Plasmid

#224144

Purpose

IPTG-inducible expression of C. elegans protein IDH-1(R133H) with a chitin tag

Depositor

Katherine Walstrom

Article

Bouchard et al Int J Mol Sci. 2025 Aug 25;26(17):8238.

Insert

IDH-1(R133H)

Tags

intein-CBD

Expression

Bacterial

Mutation

R133H

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::QF+hGR::SL2::tagBFP

Plasmid

#232607

Purpose

Pan-neuronal expression of chimeric protein 'QF+hGR' and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

QF+hGR

Expression

Worm

Mutation

N/A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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GFP H2B SL2

Plasmid

#227718

Purpose

Plasmid contains GFP H2B SL2, with some nlp-51 homology sequence after, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

GFP H2B SL2 (nlp-51 Nematode)

Use

Cloning vector

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAS3-tdc-1P::AI::acc-1::SL2::his-24::tagRFP::unc-54-3'UTR

Plasmid

#225926

Purpose

Expressing ACC-1 in RIM neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

ACC-1 (acc-1 Nematode)

Expression

Worm

Promoter

tdc-1

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAS3-tdc-1P::AI::lgc-47::SL2::his-24::tagRFP::unc-54-3'UTR

Plasmid

#225925

Purpose

Expressing LGC-47 in RIM neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

LGC-47 (lgc-47 Nematode)

Expression

Worm

Promoter

tdc-1

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAS3-npr-9P::AI::lgc-47::SL2::tagBFP::unc-54-3'UTR

Plasmid

#225923

Purpose

Expressing LGC-47 in AIB neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

LGC-47 (lgc-47 Nematode)

Expression

Worm

Promoter

npr-9

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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MDH1_KW

Plasmid

#224145

Purpose

IPTG-inducible expression of C. elegans protein MDH-1 with a chitin tag

Depositor

Katherine Walstrom

Article

Thomas et al Biochim Biophys Acta Proteins Proteom. 2

Insert

MDH-1 (F46E10.10 Nematode)

Tags

Ala-intein-CBD

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMS161

Plasmid

#215683

Purpose

Guide only plasmid targeting chrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTCCAGCGGCAGATCGGCGG

Use

CRISPR

Expression

Worm

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMS160

Plasmid

#215682

Purpose

Guide only plasmid targeting chrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTTTGAGTAGAGCACTCAGG

Use

CRISPR

Expression

Worm

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHIT182

Plasmid

#204511

Purpose

Bacterial expression of catalytically defective CSR-1a with 6xHis and maltose binding protein tags for in vitro assays

Depositor

Yuji Kohara

Article

Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.

Insert

csr-1a with D769A mutation (C. elegans) (csr-1 Nematode)

Tags

6xHis, MBP

Expression

Bacterial

Mutation

D769A

Promoter

T7lac

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wmCardinal-T2A-HO1

Plasmid

#197249

Purpose

Expresses the protein of wmCardinal-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmCardinal-T2A-HO1

Expression

Worm

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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