Addgene

We narrowed to 142,528 results for: ins

Showing: 1661 - 1680 of 142528 results

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pDM1081

Plasmid

#108981

Purpose

Knockout cassette Hygromycin

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

Knockout cassette Hygromycin

Use

Cre/Lox; Dictyostelium discoideum knockout vector

Tags

Expression

Mutation

Promoter

coaA

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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HA-RNF4/pRK5

Plasmid

#59743

Purpose

Expresses RNF4 in mammalian cells

Depositor

Xiaolu Yang

Article

Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

Insert

RNF4 (RNF4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr

Plasmid

#19326

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pmyo-3::twk-18(cn110)cDNA

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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Stat2 pBluescript SK (-)

Plasmid

#8705

Depositor

Jim Darnell

Article

Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

Insert

Stat2 (STAT2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-XK

Plasmid

#184369

Purpose

Express human XK in Expi293 cells

Depositor

Karin Reinisch

Article

Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

Insert

Human XK (XK Human)

Use

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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YSBE 610 : pHYG_6xHA

Plasmid

#92091

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, HYG selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

6xHA

Use

tagging proteins in cryptococcus neoforman

Tags

Expression

Mutation

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN1839)

Plasmid

#163509

Purpose

AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq applications.

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Has Service

AAV PHP.eB

Insert

H2B-SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn1

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007)

Plasmid

#173454

Purpose

Contains mScarletI-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-I-mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-mScarlet-mNeonGreen-kanMX (pKBJ008)

Plasmid

#173455

Purpose

Contains mScarlet-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ68 - Exp5605_Punc-122::GFP::unc-54utr

Plasmid

#19325

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Punc-122::GFP

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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pSMF2

Plasmid

#112819

Purpose

Expresses wild-type Rpb1 in yeast cells

Depositor

Stephen Fuchs

Article

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pComb3HTT

Plasmid

#63900

Purpose

Backbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.

Depositor

Carlos Barbas

Article

Barbas et al Methods, A companion to Methods in Enzym

Insert

TT Fab

Use

Phage display

Tags

geneIII

Expression

Bacterial

Mutation

Promoter

lacZ

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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IBMc090

Plasmid

#161937

Purpose

Carries engineered transposon for IBM

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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p6643 MSCV-P C-FlagHA 31E7-Kozak

Plasmid

#35021

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV31 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only
pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)

Plasmid

#173456

Purpose

Contains mScarletH-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-H-mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-MCP1

Plasmid

#184341

Purpose

Express yeast MCP1 in Expi293F cells

Depositor

Karin Reinisch

Article

Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

Insert

Yeast MCP1 (MCP1 Budding Yeast)

Use

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDX_init containing Sb-loop

Plasmid

#132697

Purpose

pDX_init plasmid containing a loop Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_loop

Use

M13 phagmid vector

Tags

Expression

Mutation

Promoter

lac

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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p6647 MSCV-P C-Flag HA 2aE7

Plasmid

#35137

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV2a E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
p6649 MSCV-P C-FlagHA 38 E7-Kozak

Plasmid

#35143

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV38 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
p6656 MSCV-P C-FlagHA 25E7-Kozak

Plasmid

#35022

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV25 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only