We narrowed to 4,125 results for: spl

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pCAG-nucGFP11-3xControlgRNA

Plasmid

#224568

Purpose

Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

GFP11-H2B

Use

CRISPR

Tags

Histone H2B (nuclear localization)

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMS158

Plasmid

#215679

Purpose

Empty repair plasmid for ChrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

5'HA + MCS + lox2272 + rps-0p::5'ΔHygR

Use

CRISPR and Cre/Lox

Expression

Worm

Mutation

5' ∆HYGR encodes aa1-226

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTKEI-Dest-loxP-sfGFP

Plasmid

#202715

Purpose

Empty expression vector containing Cre recombinase reporter.

Depositor

Mary Dunlop

Article

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

Pw7-loxP-term-loxP-sfGFP

Use

Cre/Lox

Expression

Bacterial

Promoter

constitutive

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

Plasmid

#172066

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-bJun-C-mSOG-IRES-mCherry

Plasmid

#136600

Purpose

Encodes bJun domain tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

Depositor

John Ngo

Article

Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

Insert

bJun-C-mSOG

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-mSOG-bFos-IRES-mCherry

Plasmid

#136599

Purpose

Encodes bFos domain tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).

Depositor

John Ngo

Article

Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

Insert

N-mSOG-bFos

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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P16ΔS:(MCS)-3xHA-S11

Plasmid

#108185

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

P16ΔS

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS 3xFLAG Mbnl2

Plasmid

#217032

Purpose

Overexpression of Mbnl2 in mammalian cells

Depositor

Chaolin Zhang

Article

Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

Insert

Mbnl2 (Mbnl2 Mouse)

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-WWC3

Plasmid

#172996

Purpose

constitutive expression of FLAG-tagged WWC3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-WWC3 (WWC3 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NODAL

Plasmid

#115638

Purpose

For targeted integration and inducible expression of human NODAL using doxycycline

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MyD88-NpLxIS

Plasmid

#131347

Purpose

Expresses MyD88 with 2 copies of pLxIS motif from STING fused to its N-ternimus

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

MyD88-NpLxIS (Myd88 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSG5-mERRbeta-short

Plasmid

#52188

Purpose

Expresses mouse ERRbeta short form splice variant in mammalian cells

Depositor

Rebecca Riggins

Article

Heckler et al Cell Cycle. 2014 Dec 12:0.

Insert

Estrogen-related receptor beta (Esrrb Mouse)

Expression

Mammalian

Promoter

SV40

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-Site6

Plasmid

#24515

Depositor

Kazuo Kinoshita

Article

Uemura et al PLoS One. 2010 . 5(3):e9846.

Insert

Split RFP-GFP chimera

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

May 26, 2010

Availability

Academic Institutions and Nonprofits only

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pENTR-Site5

Plasmid

#24514

Depositor

Kazuo Kinoshita

Article

Uemura et al PLoS One. 2010 . 5(3):e9846.

Insert

Split GFP-RFP chimera

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

May 5, 2010

Availability

Academic Institutions and Nonprofits only

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pGEX-UAP56

Plasmid

#157661

Purpose

Expresses UAP56 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Spliceosome RNA helicase DDX39B (DDX39B Human)

Tags

GST tag

Expression

Bacterial

Mutation

deleted amino acids 1-43

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pHBS1410 GFP-TDP43 F-S

Plasmid

#118799

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Mutation

All F to S in CTD of full-length TDP43

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET23a_mod_RBPMS_FL

Plasmid

#59393

Purpose

Plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

6xHis, FLAG, and HA

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO-tet-puro-sh-ex16b

Plasmid

#35168

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pCMV7.1_3xFLAG-STK3

Plasmid

#172990

Purpose

constitutive expression of FLAG-tagged STK3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-STK3 (STK3 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSPdH45

Plasmid

#199767

Purpose

Expresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSPdH45

Tags

His6-Tag

Expression

Bacterial

Mutation

internal His-Tag

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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