164,577 results

1. sgTP53_3

   Plasmid

   #78164

   Purpose

   sgTP53

   Depositor

   William Hahn

   Article

   Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pSynSRE-T-Luc

   Plasmid

   #60444

   Purpose

   Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

   Depositor

   Timothy Osborne

   Article

   Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

   Insert

   HMG-CoA synthase promoter (-324/-225)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

   Available Since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXS-GshF-blasticidin

   Plasmid

   #210313

   Purpose

   Expresses humanized Streptococcus thermophilus GshF in mammalian cells

   Depositor

   Kivanc Birsoy

   Article

   Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

   Insert

   Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
   

   Use

   Retroviral

   Tags

   FLAG peptide

   Expression

   Mutation

   Codon optimized for expression in human cells

   Promoter

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. GST-RNF126

   Plasmid

   #138643

   Purpose

   Bacterial expression of GST-RNF126 fusion protein

   Depositor

   Jane McGlade

   Article

   Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

   Insert

   RNF126 (RNF126 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_PGK_LaG16_2_synNotch_Gal4VP64

   Plasmid

   #85421

   Purpose

   Lentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic Notch

   Depositor

   Wendell Lim

   Article

   Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

   Insert

   LaG16_2 GFP nanobody synNotch Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-TadA8e-PcrA M6

   Plasmid

   #221229

   Purpose

   Express HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   TadA8e-PcrA M6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330A-nBRD4/PITCh

   Plasmid

   #91794

   Purpose

   PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken β-actin promoter

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CW3SL-EGFP

   Plasmid

   #61463

   Purpose

   AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-SLCO2B1_STOP

   Plasmid

   #161032

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLCO2B1 (SLCO2B1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. LSB-hsa-let-7f-5p

    Plasmid

    #103156

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-let-7f-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-let-7f-5p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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