172,049 results

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pCDNA3-Flag-SPRTN-wt

Plasmid

#110214

Purpose

Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

Depositor

Ivana Marinovic-Terzic, Kristijan Ramadan

Article

Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

Insert

SPRTN (SPRTN Human)

Expression

Mammalian

Mutation

P296L (see depositor comments below)

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA-wt_mScarlet-i_SGFP2

Plasmid

#85071

Purpose

Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGBKT7-GW

Plasmid

#61703

Purpose

Gateway compatible Y2H vector - BD

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pHIS3p:yomWasabi-Tub1+3'UTR::HPH

Plasmid

#50631

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

yomWasabi-Tub1

Use

Yeast expression

Tags

yomWasabi

Promoter

HIS3p

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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VP12

Plasmid

#72247

Purpose

Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A and Q926A

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCI-Myc3-HPS4

Plasmid

#133931

Purpose

Expresses 3xMyc-HPS4 construct in mammalian cells

Depositor

Juan Bonifacino

Article

Martina et al J Biol Chem. 2003 Aug 1;278(31):29376-84

Insert

HPS4 (HPS4 Human)

Tags

3xMyc tag

Expression

Mammalian

Mutation

Glutamic acid 229 to Glycine; Valine 552 to Methi…

Promoter

CMV I.E.

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2114

Plasmid

#49139

Purpose

AAV plasmid with FEV (Ple67) promoter driving expression of EmGFP.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple67-emGFP

Use

AAV

Expression

Mammalian

Promoter

FEV

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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HA Depdc5 pRK5

Plasmid

#46327

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Depdc5 (DEPDC5 Human)

Tags

HA

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
HA-HIF1a (S668A)

Plasmid

#49463

Purpose

HA-tagged HIF-1a Ser668 resuide mutated to Ala

Depositor

Wafik El-Deiry

Article

Warfel et al Cell Cycle. 2013 Oct 25;12(23).

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 668 to Alanine

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only
pRB2960

Plasmid

#8677

Depositor

David Botstein

Article

Anders et al Mol Biol Cell 2001 Dec;12(12):3973-86.

Insert

GFP::TUB1 under GAL1 promoter (TUB1 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only