162,199 results

1. pGEX6P1-N-FLAG

   Plasmid

   #119759

   Purpose

   Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.

   Depositor

   Martin Reijns, Andrew Jackson

   Type

   Empty backbone

   Use

   Tags

   FLAG and GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-IP HA-Parkin

   Plasmid

   #38248

   Depositor

   Noboru Mizushima

   Article

   Yoshii et al J Biol Chem. 2011 Jun 3;286(22):19630-40

   Insert

   Parkin (PRKN Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKI_ΩBBMVP16&WUS

   Plasmid

   #220348

   Purpose

   Expresses BBM-VP16 and WUS in plant cells

   Depositor

   Tomoko Igawa

   Article

   Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.

   Inserts

   BABY BOOM (BBM Mustard Weed)
   WUSCHEL (WUS Mustard Weed)
   

   Use

   Tags

   VP16 transcriptional activation domain

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S and RPS5A

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-hNurr1-NE

   Plasmid

   #102363

   Purpose

   Overexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cells

   Depositor

   Shu Leong Ho

   Insert

   NR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
   

   Use

   Tags

   NE

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-Neo-TRE-CMV-Cre-rtTA

   Plasmid

   #165457

   Purpose

   Tetracycline inducible CRE expression from AAVS1 locus

   Depositor

   Madeline Lancaster

   Article

   Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

   Insert

   CRE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tight TRE promoter

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_MMP-9-HA

   Plasmid

   #121172

   Purpose

   Expression of mouse MMP9

   Depositor

   M. Elizabeth Fini, Shinwu Jeong

   Article

   Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

   Insert

   MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   CMV immediate/early

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG-AausFP1-Z1

   Plasmid

   #135047

   Purpose

   AausFP1 - bright green fluorescent protein expression from A. australis under CAG promoter in minimal backbone plasmid.

   Depositor

   Jordan Green

   Insert

   AausFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG Promoter

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRsetB-PercevalHR

   Plasmid

   #49081

   Purpose

   Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

   Depositor

   Gary Yellen

   Article

   Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

   Insert

   PercevalHR
   

   Use

   Tags

   7xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. NL4-3 mCherry Luciferase

   Plasmid

   #44965

   Purpose

   HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.

   Depositor

   Warner Greene

   Article

   Lassen et al PLoS One. 2012;7(1):e30176. doi: 10.1371

   Insert

   mCherry, Luciferase, IRES
   

   Use

   Lentiviral

   Tags

   Luciferase, IRES and mCherry with D212G

   Expression

   Mutation

   S34C mutation in Nef

   Promoter

   Available since

   Oct. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-R-Rac1

    Plasmid

    #180576

    Purpose

    ddRFP sensor of Rac1

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    ddRFP sensor of Rac1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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