164,577 results

1. pTubb3-MC

   Plasmid

   #87112

   Purpose

   minicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP SMG1 WT

   Plasmid

   #199571

   Purpose

   Expresses WT SMG1 fused to GFP

   Depositor

   Michael Kastan

   Article

   McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

   Insert

   SMG1 WT (SMG1 Human)
   

   Use

   Tags

   GFP fusion protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNUT N6His hTFNG

   Plasmid

   #67240

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin in mammalian cells

   Depositor

   Anne Mason, Ross MacGillivray

   Article

   Mason et al Biochemistry. 2002 Jul 30;41(30):9448-54

   Insert

   human serum transferrin (TF Human)
   

   Use

   Tags

   N-terminal signal peptide, 4 aa link, 6 His, Fact…

   Expression

   Mammalian

   Mutation

   Asn413 Asp, Asn611Asp

   Promoter

   SV40

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. CP1041-ABE8e

   Plasmid

   #138493

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSpCas9 (CP1041)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmScarlet_NES_C1

   Plasmid

   #85060

   Purpose

   In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMG-RIS

   Plasmid

   #225132

   Purpose

   For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Km.

   Depositor

   Lars Blank

   Article

   Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. LSB-hsa-let-7f-1-3p

   Plasmid

   #103154

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-let-7f-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-let-7f-1-3p target (MIRLET7F1 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. BcLOV4-mammalian-recoded_mCherry_pcDNA3.1

   Plasmid

   #114595

   Purpose

   Encodes mCherry-tagged BcLOV4 for expression in mammalian cells

   Depositor

   Brian Chow

   Article

   Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

   Insert

   BcLOV4
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PyronicSF-mRuby2/pBI-CMV1

   Plasmid

   #124830

   Purpose

   Highly resposive GFP-based pyruvate nanosensor.

   Depositor

   Luis Felipe Barros

   Article

   Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

   Inserts

   PyronicSF
   mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTXB1-pA-DddA11_NT

    Plasmid

    #232443

    Purpose

    ProteinA-DddA11_NT for D&Dseq (sc TFs molecular footprint)

    Depositor

    Ivan Raimondi

    Article

    Chi et al bioRxiv [Preprint]. 2025 Jan 2:2024.12.3

    Insert

    pA-DddA_NT
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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