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171,128 results

Showing: 8431 - 8440 of 171128 results

  1. pCJ190

    Plasmid
    #162667
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162667-plasmid-map-sequence-id-321443

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  3. pNL [NlucP/IFNb/Hygro] Vector

    Plasmid
    #236928
    Purpose
    Express IFNb in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    IFNb (IFNB1 Human)
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Mutation
    None
    Available Since
    Aug. 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236928-plasmid-map-sequence-id-474672

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  4. pT2/SV-Venus-SIINFEKL

    Plasmid
    #111624
    Purpose
    Sleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studies
    Depositor
    Jon Yewdell
    Insert
    Venus-LEQLE-SIINFEKL-TEW
    Tags
    LEQLE-SIINFEKL-TEW
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111624-plasmid-map-sequence-id-213225

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  5. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Article
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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  6. pcDNA3.1-3'TwStrep

    Plasmid
    #208618
    Purpose
    Empty vector for mammalian expression, insert Twin Strep tag using BamHI and AflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with Twin Strep tag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Twin Strep
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208618-plasmid-map-sequence-id-418314

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Showing: 8431 - 8440 of 171128 results