We narrowed to 14,585 results for: pET

1. pET303C-hSOD1 A4V/C6S

   Plasmid

   #139667

   Purpose

   Expression plasmids containing human A4V/C6S SOD1

   Depositor

   Svetlana Antonyuk

   Article

   Chantadul et al Commun Biol. 2020 Mar 5;3(1):97. doi: 10

   Insert

   Superoxide dismutase-1 (SOD1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   change alanine 4 to valine, change cysteine 6 to …

   Promoter

   T7

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro

   Plasmid

   #169665

   Purpose

   Tier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK / RPBSA

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVH230-2-Tier1-PmPGK1-YPet

   Plasmid

   #169521

   Purpose

   Tier-1 vector encoding PmPGK1-driven YPet expression (PmPGK1-YPet-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven yellow fluorescent protein Ypet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTS2388-Tier1-PhCMV-p2a_Ypet

   Plasmid

   #169525

   Purpose

   Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet fused to a "self-cleaving" peptide p2a (PhCMV-p2a_YPet-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P2A-fused yellow fluorescent protein Ypet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFOX8-Tier1-PhCMV-YPet

   Plasmid

   #169506

   Purpose

   Tier-1 vector encoding PhCMV-driven YPet expression (PhCMV-YPet-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven yellow fluorescent protein Ypet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 3020_pETcon-SARS-CoV-2-RBD_N501Y

   Plasmid

   #184405

   Purpose

   yeast surface display of the SARS-CoV-2 Alpha variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Alpha Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   N501Y

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28_mouse-Caspase-1-H340D_enzymatic-domain

   Plasmid

   #183388

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1 (AA130-404)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   H340D

   Promoter

   T7

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28_canine-Caspase-1/4_enzymatic-domain

   Plasmid

   #183383

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1/4 (AA130-404) (CASP4 Canis lupus familiaris)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pETM-60-NusA-DmGe1_1220-1354_G

   Plasmid

   #146420

   Purpose

   Bacterial Expression of DmGe1_1220-1354

   Depositor

   Elisa Izaurralde

   Article

   Jinek et al RNA. 2008 Oct . 14(10):1991-8.

   Insert

   DmGe1_1220-1354 (Ge-1 Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETM-60-NusA-DmEDC3_331-440_H

    Plasmid

    #146448

    Purpose

    Bacterial Expression of DmEDC3_331-440

    Depositor

    Elisa Izaurralde

    Insert

    DmEDC3_331-440 (Edc3 Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-UTX(420-633)-H6

    Plasmid

    #183773

    Purpose

    Express pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.

    Depositor

    Peter Harte

    Article

    Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

    Insert

    UTX
    

    Use

    Tags

    6 His tag at the C-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETM-30-GST-DmBelle_695-798_B

    Plasmid

    #145961

    Purpose

    Bacterial Expression of DmBelle_695-798

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle_695-798 (bel Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETM-60-NusA-DmBelle_1-298_B

    Plasmid

    #145964

    Purpose

    Bacterial Expression of DmBelle_1-298

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle_1-298 (bel Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETM-60-NusA-DmBelle_695-798_B

    Plasmid

    #145965

    Purpose

    Bacterial Expression of DmBelle_695-798

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle_695-798 (bel Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETM-11-6His-DmBelle_1-694_B

    Plasmid

    #145951

    Purpose

    Bacterial Expression of DmBelle_1-694

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle_1-694 (bel Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETM-11-6His-DmBelle_299-694_B

    Plasmid

    #145952

    Purpose

    Bacterial Expression of DmBelle_299-694

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle_299-694 (bel Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETM-11-6His-DmBelle_299-798_B

    Plasmid

    #145953

    Purpose

    Bacterial Expression of DmBelle_299-798

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle_299-798 (bel Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETM-11-6His-DmBelle_695-798_B

    Plasmid

    #145954

    Purpose

    Bacterial Expression of DmBelle_695-798

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle_695-798 (bel Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETM-30-GST-DmBelle_1-298_B

    Plasmid

    #145957

    Purpose

    Bacterial Expression of DmBelle_1-298

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle_1-298 (bel Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM-30-GST-DmBelle_1-694_B

    Plasmid

    #145958

    Purpose

    Bacterial Expression of DmBelle_1-694

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle_1-694 (bel Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pETM-30-GST-DmBelle_299-694_B

    Plasmid

    #145959

    Purpose

    Bacterial Expression of DmBelle_299-694

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle_299-694 (bel Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pETM-30-GST-DmBelle_299-798_B

    Plasmid

    #145960

    Purpose

    Bacterial Expression of DmBelle_299-798

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle_299-798 (bel Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pETM-30-GST-DmGW182_1-539_F

    Plasmid

    #146264

    Purpose

    Bacterial Expression of DmGW182_1-539

    Depositor

    Elisa Izaurralde

    Insert

    DmGW182_1-539 (gw Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one non silent mutation G349A (G117R) compared to…

    Promoter

    Available since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a-anticalin (E60amb)-His

    Plasmid

    #168043

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Glu 60 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28-sfGFP-DogTag Loop C

    Plasmid

    #171781

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp173 and Gly174

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-DogTag Loop C
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag and linkers inserted between residues Asp1…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28-sfGFP-SpyTag003 Loop B

    Plasmid

    #171777

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp102 and Asp103

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-SpyTag003 Loop B
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    SpyTag003 and linkers inserted between residues A…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28-sfGFP-SpyTag003 Loop C

    Plasmid

    #171778

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp173 and Gly174

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-SpyTag003 Loop C
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    SpyTag003 and linkers inserted between residues A…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a-anticalin (E143amb)-His

    Plasmid

    #168046

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Glu 143 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a-anticalin (S87amb)-His

    Plasmid

    #168044

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Ser 87 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a-anticalin (N116amb)-His

    Plasmid

    #168045

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Asn 116 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28a-anticalin (N21amb)-His

    Plasmid

    #168041

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Asn 21 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET15b p97-His D592Amb

    Plasmid

    #169022

    Purpose

    Expresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteria

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p97
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    D592TAG

    Promoter

    T7

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28a-anticalin (I55amb)-His

    Plasmid

    #168042

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Ile 55 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pETCON-Aga2p-TAPBPR-myc

    Plasmid

    #153486

    Purpose

    Yeast expression of Aga2p-TAPBPR-myc

    Depositor

    Erik Procko

    Article

    McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

    Insert

    TAPBPR (TAPBPL Human)
    

    Use

    Tags

    Aga2P and c-myc epitope tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET19b-rDAD2 cPGFP(N180)

    Plasmid

    #166001

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rDAD2 cPGFP(N180)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LSSmOrange- V49A (please see depositor comments)

    Promoter

    T7 promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET19b-DAD2 cPGFP(A149)

    Plasmid

    #165998

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    DAD2 cPGFP(A149)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET15b/GVcl 1-1066 /*TBM

    Plasmid

    #162781

    Purpose

    amplification of Gallus gallus Vcl mutated in Tankyrase Binding Domain II

    Depositor

    Laura Lafon-Hughes

    Article

    et al PeerJ

    Insert

    Gallus gallus vinculin (VCL Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed Gly 454 to Val

    Promoter

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Pet22b-mEGFP-YAP-C-IDR

    Plasmid

    #166441

    Purpose

    Bacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion protein

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, YAP-C-IDR(266-504) (YAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET19b-rShHTL7 cPGFP(E167)

    Plasmid

    #166004

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rShHTL7 cPGFP(E167)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET19b-rShHTL7 cPGFP(D165)

    Plasmid

    #166003

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rShHTL7 cPGFP(D165)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge