We narrowed to 11,424 results for: 112

1. pUC18-mini-Tn7T-Lac-dCas9 (Ptac)

   Plasmid

   #105234

   Purpose

   Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescens

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Bacterial

   Mutation

   D10A, H599A

   Promoter

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGAN230

   Plasmid

   #131122

   Purpose

   reverse yTRAP, RNQ1-aTF reporting on tetR-mKate2

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SUP35

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. prCAG-MS2

   Plasmid

   #191121

   Purpose

   Transcription of 47xCAG and 12xMS2 repeats from a tetracycline-inducible promoter

   Depositor

   Ariel Lindner

   Article

   Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

   Inserts

   rCAG
   MS2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Tet

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJV53-Cas12a

   Plasmid

   #158706

   Purpose

   CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   FnCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetO

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-NLS-ZapCV2-ALA

   Plasmid

   #203665

   Purpose

   Expresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cells

   Depositor

   Amy Palmer

   Article

   Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

   Insert

   ZapCV2-Ala zinc dead sensor
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Cys and His in Zap1 is mutated with Ala

   Promoter

   CMV promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPN-mKate (PT7LacO-PN-mKate)

   Plasmid

   #191126

   Purpose

   Expression of protein N tagged with mKate from a T7-lac inducible promoter

   Depositor

   Ariel Lindner

   Article

   Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

   Insert

   Protein N
   

   Use

   Synthetic Biology

   Tags

   mKate

   Expression

   Mutation

   Promoter

   T7-lac inducible promoter

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC18-mini-Tn7T-Plac-dCas9 (Plac)

   Plasmid

   #105235

   Purpose

   Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosa

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Bacterial

   Mutation

   D10A, H599A

   Promoter

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRTC2-puro

   Plasmid

   #190567

   Purpose

   Expresses the full-length human L1 (L1.3) retrotransposon and an engineered neomycin retrotransposition reporter to monitor retrotransposition efficiency in cultured mammalian cells.

   Depositor

   Anthony Furano

   Article

   Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   L1 (L1.3) with reporter cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-UBC-MCP-mNeonGreen

   Plasmid

   #207581

   Purpose

   The lentiviral plasmids for expression of MCP-NeonGreen

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   MS2 coat protein mNeonGreen fusion
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   UBC

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-loxP-GRAB_DA2m-loxP

    Plasmid

    #189751

    Purpose

    Excludes sensor expression in Cre-positive neurons

    Depositor

    Yulong Li

    Article

    Dai et al Cell Rep. 2022 Aug 23;40(8):111246. doi:

    Insert

    Dopamine sensor GRAB_DA2m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRANS_213

    Plasmid

    #91112

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: WDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmyc-GFP-TNRC6B

    Plasmid

    #42022

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6B (TNRC6B Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST-mCherry-LacR-PALB2

    Plasmid

    #71112

    Purpose

    mammalian expession of mCherry-LacR-PALB2

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    PALB2 (PALB2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCerulean-hCOL1A1

    Plasmid

    #140112

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PROM5_35Sx2

    Plasmid

    #136112

    Purpose

    Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM_35Sx2 (CaMV)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pTRANS_231

    Plasmid

    #91120

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: BeYDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAC-crRNA-Km

    Plasmid

    #158712

    Purpose

    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    AcGFP (for crRNA cloning)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mApple-ERM

    Plasmid

    #217377

    Purpose

    ER marker

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    rat cb5 ERM
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRT801

    Plasmid

    #68112

    Purpose

    Integration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.

    Depositor

    Maggie Smith

    Article

    Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

    Inserts

    aac3(IV)
    oriT
    attP
    int
    

    Use

    Streptomyces integrating vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFGC5941-PacI-ath-STTM2937

    Plasmid

    #84112

    Purpose

    target miRNA2937 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM2937
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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