We narrowed to 4,681 results for: POR C

1. MLF2-GFP-3xFLAG

   Plasmid

   #230986

   Purpose

   Expresses GFP tagged MLF2 in mammalian cells

   Depositor

   Christian Schlieker

   Article

   Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

   Insert

   MLF2 (MLF2 Human)
   

   Use

   Tags

   3xFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1alpha-soCoChR-tdTomato

   Plasmid

   #107714

   Purpose

   A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

   Depositor

   Edward Boyden

   Article

   Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

   Insert

   soCoChR-tdTomato
   

   Use

   AAV

   Tags

   KA2 and tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMKII-soCoChR-GFP

   Plasmid

   #107711

   Purpose

   A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CaMKII promoter

   Depositor

   Edward Boyden

   Article

   Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

   Insert

   soCoChR-GFP
   

   Use

   AAV

   Tags

   EGFP and KA2

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221_SLC39A8

   Plasmid

   #132177

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

   Insert

   SLC39A8 (SLC39A8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pX330-sgRNA_Ago2_1

   Plasmid

   #73529

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pX330-sgRNA_Ago2_2

   Plasmid

   #73530

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-CAG-soCoChR-GFP

   Plasmid

   #107710

   Purpose

   A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CAG promoter

   Depositor

   Edward Boyden

   Article

   Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

   Insert

   soCoChR-GFP
   

   Use

   AAV

   Tags

   EGFP and KA2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-soCoChR-mCardinal

   Plasmid

   #107713

   Purpose

   A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. hSynapsin promoter

   Depositor

   Edward Boyden

   Article

   Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

   Insert

   soCoChR-mCardinal
   

   Use

   AAV

   Tags

   KA2 and mCardinal

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Hy_pMT Flag-Hel25E K91N

   Plasmid

   #110121

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   K91N mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Hy_pMT Flag-Hel25E D193E

    Plasmid

    #110120

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    D193E mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSET a sfMatryoshCaMP6s-T78H

    Plasmid

    #100021

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfMatryoshCaMP6s
    

    Use

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP and T78H m…

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1alpha-soCoChR-GFP

    Plasmid

    #107709

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Insert

    soCoChR-GFP
    

    Use

    AAV

    Tags

    EGFP and KA2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SLC39A5

    Plasmid

    #132057

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

    Insert

    SLC39A5 (SLC39A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pKT2/CAGXSP/GFPCD63

    Plasmid

    #231709

    Purpose

    Stably express CD63 fused with GFP in mammalian cells via the sleeping beauty transposon system.

    Depositor

    Masamitsu Kanada

    Article

    Li et al Sci Rep. 2024 Nov 9;14(1):27432. doi: 10

    Insert

    CD63 (CD63 Human)
    

    Use

    Tags

    gfp

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGGI_Myr-YFP-MiniTurboID

    Plasmid

    #222432

    Purpose

    Potential plasma membrane control.

    Depositor

    Dominique Bergmann

    Article

    Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

    Insert

    MiniTurboID (BirA mutant)
    

    Use

    Golden gate / green gate compatible cloning vector

    Tags

    Linker, Myr, and YFP

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330_SAFB2gRNA12

    Plasmid

    #196106

    Purpose

    Contains guide RNA to 3' end of mouse SAFB2 gene for safb1/2 dko. Used with Addgene IDs: 196103, 196107, 196108

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB2 (Safb2 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330_SAFB2gRNA13

    Plasmid

    #196107

    Purpose

    Contains guide RNA to 3' end of mouse SAFB2 gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196108

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB2 (Safb2 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pS238D1::sptse5-CT

    Plasmid

    #192959

    Purpose

    For expression of Tse5-CT (1169-1317) in P. putida with the PelB signal peptide fused at the N-terminus in biological function studies

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    sptse5-CT
    

    Use

    Tags

    PelB signal peptide

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1317)

    Promoter

    Available Since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. myc-Miro2 (lacking TM)

    Plasmid

    #127614

    Purpose

    Human Miro2 with the transmembrane deleted

    Depositor

    Josef Kittler

    Article

    Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

    Insert

    Miro2 (RHOT2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Transmembrane deletion

    Promoter

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHTSHP-ShhN-Cys

    Plasmid

    #121136

    Purpose

    Expresses ShhN with a C-terminal cysteine residues in bacteria

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    shh (Shh Mouse)
    

    Use

    Tags

    HIS, HRV3C site, and MBP

    Expression

    Bacterial

    Mutation

    Amino acid sequence from 26-191, with an addition…

    Promoter

    tac

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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