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pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4

Plasmid

#193345

Purpose

tetO-OS*K (Dox-inducible reprogramming lentiviral OSK vector expressing mouse Oct4 + super-Sox + Klf4)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Oct4-p2a-Sox2-17-t2a-Klf4 (Klf4, Pou5f1, Sox2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

TetO mini CMV

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Oct4-p2a-Sox2AV-t2a-Klf4-e2a-Myc

Plasmid

#193347

Purpose

tetO-Sox2AV-KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Sox2A61V + Klf4 + cMyc)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Oct4-p2a-Sox2AV-t2a-Klf4-e2a-cMyc (Klf4, Sox2, Pou5f1 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

Sox2A61V is a highly cooperative point mutant of …

Promoter

TetO mini CMV

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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PB-TAC-EB-C5

Plasmid

#80485

Purpose

piggyBac transposon for dox-inducible expression of the EB-C5 polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

lin-28 homolog A (C. elegans) (Pou5f1, Sox2, Klf4, Myc, Lin28a Mouse)

Use

Piggybac transposon

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-Zim3-MeCP2

Plasmid

#220143

Purpose

doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression

Depositor

Frank Buchholz

Article

Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii

Insert

dCas9-Zim3-MeCP2

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

Tre-3G

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pINDUCER20_mStrawberry

Plasmid

#161734

Purpose

Doxycycline-inducible expression of mStrawberry

Depositor

Alec Kimmelman

Article

Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

Insert

mStrawberry

Use

Lentiviral; Destinatioin vector for gateway cloni…

Expression

Mammalian

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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PB_tre_dCas9_KRAB

Plasmid

#126030

Purpose

PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

dCas9 fusion with KRAB
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

EF1 alpha and TRE

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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PB_iETV2_P2A_GFP_Puro

Plasmid

#168805

Purpose

PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

ETV2 (ETV2 Human, Synthetic)

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Tfap2c

Plasmid

#70269

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Tfap2c

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

transcription factor AP-2, gamma (Tfap2c Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-TREG3G-PE2-rtTA3G-P2A-eGFP

Plasmid

#196971

Purpose

Piggybac vector with doxycyclin-inducible prime editor for mammalian cells

Depositor

Leopold Parts

Article

Koeppel et al Nat Biotechnol. 2023 Feb 16. doi: 10.103

Inserts

Cas9-RT
rtTA3G-P2A-eGFP

Expression

Mammalian

Promoter

PGK and TREG3G

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-VEGFA EF1a-rtTA3

Plasmid

#195881

Purpose

Lentiviral construct of doxycycline inducible VEGFA

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

VEGFA (Vegfa Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3GS

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLIX403_Capture1_APOBEC_HA_P2A_mRuby

Plasmid

#183901

Purpose

Inducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

Capture1_APOBEC_HA_P2A_mRuby

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pN251

Plasmid

#188471

Purpose

Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

enCas12a-HF (gift from Dr. Keith Joung)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-L-MYC

Plasmid

#139817

Purpose

doxycycline-inducible expression of Human L-MYC in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

L-MYC (MYCL Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLVXT-mTcf3

Plasmid

#121935

Purpose

doxycycline-inducible mouse Tcf3

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Transcription Factor 3 (Tcf3 Mouse)

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PB-Lmo2

Plasmid

#60666

Purpose

PiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Lmo2 (Lmo2 Mouse)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Pu.1

Plasmid

#139839

Purpose

doxycycline-inducible expression of mouse Pu.1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Pu.1 (Spi1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-LUC

Plasmid

#218630

Purpose

This plasmid contains luciferase under doxycycline inducible control.

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

LUC (LOC116160065 )

Use

Lentiviral, Luciferase, and Synthetic Biology

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_Down-Tandem Donor

Plasmid

#229785

Purpose

Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-ZNF281

Plasmid

#222923

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

ZNF281 (ZNF281 Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pT076

Plasmid

#137880

Purpose

Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA

Use

AAV

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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