Addgene

We narrowed to 142,528 results for: ins

Showing: 1681 - 1700 of 142528 results

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pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)

Plasmid

#173453

Purpose

Contains mCherry-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDM1079

Plasmid

#108979

Purpose

Knockout cassette Blasticidin

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

Knockout cassette Blasticidin

Use

Cre/Lox; Dictyostelium discoideum knockout vector

Tags

Expression

Mutation

Promoter

coaA

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6-mTFP1-KanMX6

Plasmid

#69851

Purpose

C-terminal tagging with mTFP1 using KanMX6 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-PH

Plasmid

#96948

Purpose

EGFP-PLC pleckstrin homology domain for CALI validation

Depositor

Marcel Bruchez

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

Pleckstrin homology domain

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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Pdx1-RFP puro (DM#235)

Plasmid

#15085

Depositor

Douglas Melton

Article

Rajagopal et al Science. 2003 Jan 17. 299(5605):363.

Insert

Pdx1 promoter (Pdx1 Mouse)

Use

Tags

RFP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
XE12 Xwnt-11

Plasmid

#16861

Depositor

Randall Moon

Article

Du et al Mol Cell Biol. 1995 May;15(5):2625-34. d

Insert

Xwnt-11 (wnt11b.L Frog)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pQP-CiRISB

Plasmid

#138978

Purpose

Light-controlled tri-directional targeting of endogenous proteins (cytoplasm-shifted)

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-CAAX-IRES2-iB(GFP)-NESmCherry- Q-PAS1-AsLOV2cNLS-NotI

Use

Tags

Expression

Mutation

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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YSBE 557 : pNAT_GFP

Plasmid

#92085

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; GFP tag, NAT selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

GFP

Use

tagging proteins in cryptococcus neoforman

Tags

Expression

Mutation

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-PH-dL5**

Plasmid

#96947

Purpose

Expresses EGFP-PLC pleckstrin homology domain linked to FAP for CALI validation

Depositor

Marcel Bruchez

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

Pleckstrin homology domain

Use

Tags

EGFP and dL5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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PH-dL5**

Plasmid

#96946

Purpose

PLC pleckstrin homology domain linked to FAP for CALI validation

Depositor

Marcel Bruchez

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

Pleckstrin homology domain

Use

Tags

dL5 and mCerulean

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF-KpAvs4

Plasmid

#188807

Purpose

Codon optimized KpAvs4 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

KpAvs4

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCDF-EcAvs2

Plasmid

#188804

Purpose

Codon optimized EcAvs2 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs2

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF-SeAvs3

Plasmid

#188805

Purpose

Codon optimized SeAvs3 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

SeAvs3

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF-SeAvs1

Plasmid

#188803

Purpose

Codon optimized SeAvs1 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

SeAvs1

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ MBIC5-mCer3

Plasmid

#74116

Purpose

For generating mRNA for zebrafish injection of cytoplasmic fluorogen-activating protein (FAP)

Depositor

Michael Tsang

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

MBIC5-mCer3

Use

Transgenic construct

Tags

mCerulean 3

Expression

Mutation

Promoter

Myl7 (Cmlc2)

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-sfGFP (pJJF001)

Plasmid

#173457

Purpose

C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-sfGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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YSBE 608 : pHYG_GFP

Plasmid

#92089

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; GFP tag, HYG selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

GFP

Use

tagging proteins in cryptococcus neoforman

Tags

Expression

Mutation

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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POM42-mTFP1

Plasmid

#69852

Purpose

N-terminal tagging with mTFP1 using URA3 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1 minus start and stop codon

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBS Survivin

Plasmid

#19233

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Survivin (BIRC5 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pISceI-myl7:MBIC5-mCer3

Plasmid

#74117

Purpose

Zebrafish transgenic construct

Depositor

Michael Tsang

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

MBIC5-mCer3

Use

Mrna generation for microinjection.

Tags

mCerulean 3

Expression

Mammalian

Mutation

Promoter

SP6

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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