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PME-1 S156A (inakt.mut) pcDNA-StrepIII

Plasmid

#119293

Purpose

Mammalian overexpression of StrepII-tagged full length PPME1 with mutation in the methylesterase active center

Depositor

Jukka Westermarck

Insert

PPME1 (PPME1 Human)

Use

Tags

Expression

Mammalian

Mutation

S156A

Promoter

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pVH321-1-Tier1-PhCMV-dCas9-3xNLS

Plasmid

#169595

Purpose

Tier-1 vector encoding PhCMV-driven dCas9-3xNLS expression (PhCMV-dCas9-3xNLS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

dead S.pyogenes Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2-FUS-1-526aa-V5

Plasmid

#29609

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2-FUS-1-413aa-V5

Plasmid

#29612

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-413aa (FUS Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Contains FUS aa# 1-413

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA 3.2-FUS-1-269aa-V5

Plasmid

#29610

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-269aa (FUS Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Contains FUS aa# 1-269

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA 3.2-FUS-1-373aa-V5

Plasmid

#29611

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-373aa (FUS Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Contains FUS aa# 1-373

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
S1 1029 CP ABEMax strategy 1

Plasmid

#135357

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-nSpyCas9 aa 1029

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEEF1D-FLAG (pcDNA 3.1+) LG6-1

Plasmid

#37365

Depositor

David Litchfield

Article

Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

Insert

EEF1D (EEF1D Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
pFLAG-EEF1D (pcDNA 3.1+) LG3-1

Plasmid

#37364

Depositor

David Litchfield

Article

Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

Insert

EEF1D (EEF1D Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
Neurexin-1-Beta scFv [N170A/26]

Plasmid

#199425

Purpose

Mammalian expression of Neurexin-1-Beta scFv. Derived from hybridoma N170A/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingNeurexin-1-Beta (Human) (NRXN1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Kv2.2 K+ channel scFv [N372B/1]

Plasmid

#190548

Purpose

Mammalian Expression of Kv2.2 K+ channel scFV. Derived from hybridoma N372B/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv2.2 K+ channel (Rattus norvegicus) recombinant scFV (Kcnb2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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SB52

Plasmid

#21487

Depositor

Shoumo Bhattacharya

Article

Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

Insert

Cited2 (CITED2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 23, 2009

Availability

Academic Institutions and Nonprofits only
H2B-Venus

Plasmid

#20971

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Histone cluster 1, H2bb (H2BC3 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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H2B-Orange

Plasmid

#20969

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Histone cluster 1, H2bb (H2BC3 Human)

Use

Tags

Orange

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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H2B-Strawberry

Plasmid

#20970

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Histone cluster 1, H2bb (H2BC3 Human)

Use

Tags

Strawberry

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-SRm160

Plasmid

#11305

Depositor

Phil Sharp

Article

Cheng et al Mol Cell Biol. 2006 Jan . 26(1):362-70.

Insert

SRm160 (SRRM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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HA.PKD

Plasmid

#10808

Depositor

Alex Toker

Article

Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

Insert

PKD (PRKD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

L520P

Promoter

CMV

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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Flag-Src

Plasmid

#118308

Purpose

Expression of mouse Src

Depositor

Philip Stork

Article

Schmitt et al Mol Cell. 2002 Jan;9(1):85-94.

Insert

Src (Src Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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HA.PKD.Y463F

Plasmid

#10813

Depositor

Alex Toker

Article

Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

Insert

PKD (PRKD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Y463F, inactive

Promoter

CMV

Available since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
SB53

Plasmid

#21488

Depositor

Shoumo Bhattacharya

Article

Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

Insert

Cited2 (CITED2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

aa1-65 deleted

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only