We narrowed to 2,101 results for: CO

1. qTAG-N-Puro-mNeon-ACTB

   Plasmid

   #207752

   Purpose

   Donor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEMT-Mef2c-tPT2A-GFP

   Plasmid

   #111771

   Purpose

   Bicistronic construct: Co-express two genes by 2A peptides

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Insert

   Mef2c-tPT2A-GFP (Mef2c Mouse)
   

   Use

   Cloning intermediate

   Tags

   GFP

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

   Plasmid

   #203229

   Purpose

   Cre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

   Depositor

   Adam Cohen

   Insert

   Cre-dependent, membrane-localized Optopatch
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W

   Plasmid

   #163169

   Purpose

   Lentiviral plasmid with non-targeting gRNA, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   Non-targeting
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLIK CA ROCK2

   Plasmid

   #84649

   Purpose

   Lentiviral expression of doxycycline-inducible constitutively active ROCK2 and co-expression of Venus

   Depositor

   Sanjay Kumar

   Article

   Wong et al Cancer Res. 2015 Mar 15;75(6):1113-22. d

   Insert

   ROCK2 (ROCK2 Bovine)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Constitutive Active (please see depositor comment…

   Promoter

   Available since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-Solo-mStayGold-COX8A

   Plasmid

   #227309

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the COX8A locus. For mitochondria visualization. To be co-transfected with sgRNAplasmid px330-PITCh-COX8A (Addgene #227308)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   COX8A Homology Arms flanking a mStayGold Tag (COX8A Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pp-mCAT1

   Plasmid

   #83948

   Purpose

   This plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectors

   Depositor

   Philippe Mangeot, Vincent Lotteau

   Article

   Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

   Insert

   mCAT1 (Slc7a1 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-N-Puro-moxGFP-TUBA1B

   Plasmid

   #207767

   Purpose

   Donor template for Puro-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Puro-moxGFP Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. qTAG-C-Solo-mStayGold-LAMP1

   Plasmid

   #227322

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LAMP1 Homology Arms flanking a mStayGold Tag (LAMP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGLOW31

    Plasmid

    #172698

    Purpose

    C. elegans mScarlet co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-3::mScarlet
    

    Use

    Tags

    mScarlet

    Expression

    Worm

    Mutation

    Promoter

    myo-3

    Available since

    Aug. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pLVH-EF1a-GFP-P2A-KLF4

    Plasmid

    #130694

    Purpose

    Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

    Depositor

    Kejin Hu

    Article

    Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

    Insert

    human KLF4 (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-N-Blast-3xFLAG-LMNB1

    Plasmid

    #207777

    Purpose

    Donor template for Blast-2A-3xFLAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Blast-3xFLAG Cassette (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. qTAG-N-Solo-mScarlet-LMNB1

    Plasmid

    #207771

    Purpose

    Donor template for mScarlet insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a mScarlet Tag (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. CD3-2A_pMI-LO

    Plasmid

    #153418

    Purpose

    Retroviral (MSCV) expression of all four WT CD3 subunits, co-expressed with LSSmOrange

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    murine CD3 delta-gamma-epsilon-zeta subunits (Cd3d Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p28-2iDP-puro

    Plasmid

    #88893

    Purpose

    Dual-intron DMD platform plasmid. Co-expresses DMD platform segment, firefly luciferase, puroR and mCherry. Plasmid carries phiC31 and Bxb1 attB sites.

    Depositor

    Michele Calos

    Article

    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

    Inserts

    Dual-intron DMD platform
    luciferase
    puromycin resistance enzyme
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Truncated version of the dystrophin protein (tran…

    Promoter

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-AMVpl-δWT-nAchR

    Plasmid

    #97188

    Purpose

    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co

    Depositor

    Henry Lester

    Article

    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

    Insert

    Delta nicotinic acetylcholine receptor (Chrnd Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-AMVpl-γWT-nAchR

    Plasmid

    #97187

    Purpose

    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co

    Depositor

    Henry Lester

    Article

    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

    Insert

    Gamma nicotinic acetylcholine receptor (Chrng Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. qTAG-C-mNeon-Puro-TOMM20

    Plasmid

    #207790

    Purpose

    Donor template for mNeon-2A-Puro insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TOMM20 Homology Arms flanking a mNeon-Puro Cassette (TOMM20 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. qTAG-C-mStayGold-Puro-CLTC

    Plasmid

    #227314

    Purpose

    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CLTC Homology Arms flanking a mStayGold-2A-Puro Cassette (CLTC Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. qTAG-C-Solo-mStayGold-PEX3

    Plasmid

    #227304

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the PEX3 locus. For peroxisome visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PEX3 (Addgene #227303)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    PEX3 Homology Arms flanking a mStayGold Tag (PEX3 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge