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pDisplay-GRAB-gDA3h-IRES-mCherry-CAAX

Plasmid

#208693

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3h

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO

Plasmid

#204341

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-i3/Gbetagamma ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-CDK19-W105M-IRES-ZsGreen

Plasmid

#68859

Purpose

Expresses Human CDK19 W105M Mutant

Depositor

Matthew Shair

Article

Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

Insert

Cyclin-Dependent Kinase 19 (CDK19 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

W105M

Promoter

EF1α

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)

Plasmid

#105922

Purpose

AAV-mediated gene expression from CBh promoter. Contains left AAV2 ITR with an 11bp deletion in C region and canonical right AAV2 ITR

Depositor

Marcella Patrick

Inserts

mKate2
IRES-Multiple cloning site

Use

AAV

Tags

Expression

Mutation

codon optimized

Promoter

CBh and same CBh promoter as mKate2

Available since

Feb. 15, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCSC-NGN2-IRES-GFP-T2A-Sox11

Plasmid

#90214

Purpose

To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.

Depositor

Chun-Li Zhang

Article

Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

Insert

NGN2-IRES-GFP-T2A-Sox11 (NEUROG2, Sox11 Mouse, Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-PKIalpha-IRES-nls-mRuby2

Plasmid

#63059

Purpose

Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

PKIalpha (Pkia Mouse, Synthetic)

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD S1859A

Plasmid

#169821

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

S1859A; TCCC -> AGTC silent mutations at nt527…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

Plasmid

#224536

Purpose

A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

IRES-FLAG- NLS-mScarlet-I3

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB-rDA3h-IRES-EGFP-CAAX

Plasmid

#208697

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h and a membrane-localized EGFP in mammalian cells

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA3h

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLV UBC APEX2-3xFLAG-IRES-eGFP

Plasmid

#214983

Purpose

Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

APEX2 (APX2 Glycine max (soybean))

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

UBC

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-Membrin-IRES-Puromycin

Plasmid

#134865

Purpose

Expresses EGFP-tagged Golgi apparatus marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Membrin (GOSR2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

Plasmid

#113884

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Expression

Mutation

Promoter

pHR-CMV-TetO2

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre

Plasmid

#134308

Purpose

Expresses Synaptophysin-TdTomato and also tamoxifen dependant Cre (OHT-Cre, aka Cre-ER)

Depositor

Elly Nedivi

Article

Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

Inserts

Synaptophysin (Syp Mouse, Rat)
Cre-ER

Use

Tags

TdTomato

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1a-MICB*005-IRES-ZsGreen

Plasmid

#114008

Purpose

Induces expression of human MICB allele 005 cDNA

Depositor

Kai Wucherpfennig

Article

Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

Insert

MICB*005 (MICB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB-rDA2m-IRES-EGFP-CAAX

Plasmid

#208694

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2m and a membrane-localized EGFP in mammalian cells

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA2m

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1a MICA*009-IRES-ZsGreen

Plasmid

#114007

Purpose

Induces expression of human MICA allele 009 cDNA

Depositor

Kai Wucherpfennig

Article

Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

Insert

MICA*009 (MICA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-C-Myc-DDK-IRES-Puro HNRNPQ

Plasmid

#200729

Purpose

Lentiviral expression of Flag-Tagged human HNRNPQ

Depositor

Claudio Alarcón

Insert

HNRNPQ (SYNCRIP Human)

Use

Lentiviral

Tags

Myc-DDK from backbone plasmid

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-Emerin-IRES-Hygromycin

Plasmid

#134864

Purpose

Expresses EGFP-tagged nuclear envelope marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

emerin (EMD Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-human-c-MYC-ER-mouse IRES-mcherry

Plasmid

#73570

Purpose

Mammalian retroviral expression of Myc-ER

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

cMYC (MYC Human)

Use

Retroviral

Tags

IRES-mCherry and Mouse ER

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-EZH2-PGK-Puro-IRES-GFP

Plasmid

#75125

Purpose

Retroviral expression plasmid encoding wild type human EZH2

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

KMT6 (EZH2 Human)

Use

Retroviral

Tags

None

Expression

Mutation

Promoter

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only