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Plasmid#167595PurposeExpress FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.DepositorInsertFepA
UseTagsExpressionBacterialMutationHis6 tag inserted after residue 393 in the mature…PromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS406 ATG1-ATG7-3'UTR
Plasmid#166847PurposeExpresses Atg1 with ATG7 3'UTR in yeasts.DepositorInsertATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterATG1 PromoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-G2A,C4A,C5A,C7A-RFP
Plasmid#166842PurposeExpresses a myristoylation and palmitoylation mutant Vac8-RFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7AlaDepositorInsertVAC8 (VAC8 Budding Yeast)
UseTagsRFPExpressionYeastMutationG2A, C4A, C5A, C7APromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-G2A,C4A,C5A,C7A-GFP
Plasmid#166839PurposeExpresses a myristoylation and palmitoylation mutant Vac8-GFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7AlaDepositorInsertVAC8 (VAC8 Budding Yeast)
UseTagsGFPExpressionYeastMutationG2A, C4A, C5A, C7APromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-C4A,C5A,C7A-GFP
Plasmid#166838PurposeExpresses a palmitoylation mutant Vac8-GFP in yeasts, mutations are Cys4Ala, Cys5Ala, Cys7AlaDepositorInsertVAC8 (VAC8 Budding Yeast)
UseTagsGFPExpressionYeastMutationC4A, C5A, C7APromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS-ATG1-ATG7 3'UTR(406)
Plasmid#122070PurposeExpressing ATG1 with ATG7 3'UTRDepositorInsertserine/threonine protein kinase ATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationThe native 3'UTR was replaced by ATG7 3'…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSC45
Plasmid#104819PurposeCRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-2). Also expresses Cas9 from Gmubi promoter.DepositorInsertGlyma.04g057400
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC29
Plasmid#104803PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr4g094545 (Hen1). Also expresses Cas9 from Gmubi promoter.DepositorInsertMedtr4g094545
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTC130
Plasmid#91178PurposeBeYDV T-DNA vector expressing TALENs targeting tomato ANT1, selectable marker gene removed from the backbone (template for amplification of the BaeI containing fragment to create pMOD_C0000)DepositorInsertTALENs targeting tomato ANT1
UseTALENTagsExpressionMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-D10A_1
Plasmid#91159PurposeCas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9_D10A (nickase)
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC366
Plasmid#91215Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, ribozyme processing)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0808
Plasmid#91024PurposeModule A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterEC1.2Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0408
Plasmid#91010PurposeModule A, Promoter: EC1.2, Gene: AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertAtCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterEC1.2Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0708
Plasmid#91021PurposeModule A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_H840A nickase
UseCRISPRTagsExpressionMutationH840APromoterEC1.2Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK287
Plasmid#71120PurposeProduces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (H6UctB) in E. coliDepositorInsertformyl-CoA:oxalate CoA-transferase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK347
Plasmid#71833PurposeCloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59D), and purK genesDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationchanges histidine-59 to aspartatePromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK348
Plasmid#71832PurposeCloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59N), and purK genesDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationchanges histidine-59 to asparaginePromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL 1410
Plasmid#53011PurposepLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfpDepositorInsertsDsrA sRNA
ompC
rpoS
UseSynthetic BiologyTagsT7noRBS (900bp) and gfpExpressionBacterialMutationcontains −150 to +30 bp relative to start codon a…PromoterpCon, pLlacO-1, and pLtetO-1Available sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 1035
Plasmid#52999PurposepLtetO-1:RhyB, pCon:fhlA::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
fhlA
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains −309 to +96 bp relative to start codon a…PromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1033
Plasmid#52998PurposepLtetO-1:RhyB, pCon:rpoS::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
rpoS
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains −150 and +30 bp relative to start codon …PromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only