We narrowed to 3,270 results for: actin

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NDV-HN-SpyTag

Plasmid

#160000

Purpose

Expresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

NDV-HN-SpyTag

Tags

His6-tag

Expression

Mammalian

Promoter

Chicken beta-actin promoter

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBa-FRB-3myc-Rab7

Plasmid

#64210

Purpose

Rab7 with N-terminal FRB and triple myc tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Rab7 (Rab7 Mouse)

Tags

FRB and triple myc tag (3myc)

Expression

Mammalian

Promoter

chicken Beta Actin

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP-P2A-FusionRed

Plasmid

#191108

Purpose

Mammalian cell line expression plasmids for production of EGFP and FusionRed proteins

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

pAAV-CAG-EGFP-P2A-FusionRed

Use

AAV

Tags

FusionRed

Expression

Mammalian

Mutation

See depositor comments below

Promoter

chicken β-actin promoter

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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Cav2.1 HA pCAGGS

Plasmid

#206076

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pB.GFP-KIF1A tail 395-1698

Plasmid

#134618

Purpose

Mammalian expression of mouse KIF1A 395-1698

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF1A (Kif1a Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 394 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG_EGFP-RandE3

Plasmid

#79870

Purpose

Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-RandE3

Use

Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Promoter

Beta-actin

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF1C tail 400-1103

Plasmid

#134621

Purpose

Mammalian expression of human KIF1C 400-1103

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF1C (KIF1C Human)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 399 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-Lifeact-mRuby

Plasmid

#194339

Purpose

Expresses Lifeact with mRuby which labels F-actin in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Lifeact

Tags

mRuby

Expression

Mammalian

Promoter

CMV IE94

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955

Plasmid

#134613

Purpose

Mammalian expression of rat KIF5C 378-955

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5C (Kif5c Rat)

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 377 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-KKHSaCas9-BlastR

Plasmid

#107189

Purpose

Confers constitutive expression of KKH SaCas9.

Depositor

Richard Sherwood

Article

Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

Insert

KKH SaCas9 (NEWENTRY )

Expression

Bacterial

Mutation

E782K/N968K/R1015H

Promoter

Chicken ß-Actin

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

Plasmid

#129530

Purpose

Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Inserts

codon-optimized AcrIIC3Nme
sgRNA_Rosa26

Use

AAV

Tags

FLAG/NLS

Promoter

U6 and cytomegalovirus-enhancer chicken β-actin p…

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pGBT-RP2-1

Plasmid

#31828

Depositor

Stephen Ekker

Article

Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

Type

Empty backbone

Use

Cre/Lox

Tags

GFP GM2 variant and mRFP

Promoter

beta Actin

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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tdKatushka2-VASP-5

Plasmid

#56049

Purpose

Localization: Actin/Focal Adhesions, Excitation: 586, Emission: 632

Depositor

Michael Davidson

Article

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Insert

VASP

Tags

tdKatushka2

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

Plasmid

#129532

Purpose

Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Inserts

codon-optimized AcrIIA4
sgRNA_Rosa26

Use

AAV

Tags

FLAG/NLS

Promoter

U6 and cytomegalovirus-enhancer chicken β-actin p…

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

Plasmid

#52890

Purpose

"CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.

Depositor

Iswar Hariharan

Article

Bosch et al Development. 2015 Feb 1;142(3):597-606.

Inserts

LexGAD
Gal4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LINXa3

Plasmid

#73614

Purpose

Light-induced nuclear export switch for microscopy in mammalian cells.

Depositor

Brian Kuhlman

Article

Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

Insert

LINXa3

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

Chicken B-Actin

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.CNDP1-flag.WPRE.rBG

Plasmid

#132683

Purpose

AAV plasmid encoding mouse CNDP1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Carnosine dipeptidase 1 (Cndp1 Mouse)

Use

AAV

Tags

Flag

Promoter

Chicken b-actin

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963

Plasmid

#134612

Purpose

Mammalian expression of mouse KIF5B 376-963

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5B (Kif5b Mouse)

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 375 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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p5E-6xgata2aECEbasp

Plasmid

#132968

Purpose

gata2a endothelial enhancer (x6) and basal promoter in p5E Gateway vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

gata2a endothelial enhancer (x6) with a b-actin basal promoter

Use

Gateway vector

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF3B tail 391-747

Plasmid

#134615

Purpose

Mammalian expression of mouse KIF3B 391-747

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF3B (Kif5b Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 390 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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