164,629 results

1. pDONR221-NPC1_STOP

   Plasmid

   #161461

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   NPC1 (NPC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)

   Plasmid

   #214875

   Purpose

   Empty lentiviral backbone for guide array cloning

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti PGK V5-LUC Neo (w623-2)

   Plasmid

   #21471

   Purpose

   3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Neo

   Depositor

   Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   V5-LUC
   

   Use

   Lentiviral and Luciferase

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. SypHer mt

   Plasmid

   #48251

   Purpose

   pH sensor, targeted to mitochondria

   Depositor

   Nicolas Demaurex

   Article

   Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

   Insert

   SypHer
   

   Use

   Tags

   2 mitochondrial targeting sequences

   Expression

   Mammalian

   Mutation

   C199S

   Promoter

   CMV

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

   Plasmid

   #175547

   Purpose

   Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag7 and Tomm20-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pV1524

   Plasmid

   #111431

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNCS-Antares

   Plasmid

   #74279

   Purpose

   Constitutive expression of Antares in bacteria

   Depositor

   Michael Lin

   Article

   Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   Antares
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAT001

   Plasmid

   #171061

   Purpose

   Plasmid for expressing HIV-1 envelope glycoprotein

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Insert

   HIV-1 Env
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCherry-N1-cofilin

   Plasmid

   #186750

   Purpose

   Expresses wildtype mCherry-tagged human cofilin in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

   Insert

   Cofilin-1 (CFL1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBR322-Pdp1_NTD-Cas9

    Plasmid

    #198275

    Purpose

    For loading SpCas9 into PVCs; also contains PVC regulatory genes

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1_NTD-Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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