166,289 results

1. pMevT

   Plasmid

   #17815

   Depositor

   Jay Keasling

   Article

   Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW57-MCS1-P2A-MCS2 (Blast)

   Plasmid

   #80921

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAS9cd

   Plasmid

   #141252

   Purpose

   Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Article

   Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   TEF1 (Cas9), pSNR52 (gRNA)

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET16better_H10_HRV3C_chitinase

   Plasmid

   #175091

   Purpose

   Expresses B. licheniformis chitinase with an N terminal 10X His tag and HRV-3C cleavage site in E. coli.

   Depositor

   Jay C Dunlap

   Article

   Bartholomai et al Fungal Genet Biol. 2021 Aug 20;156:10361

   Insert

   chitinase
   

   Tags

   10X His, HRV-3C

   Expression

   Bacterial

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBItetDT240GFP

   Plasmid

   #96904

   Purpose

   tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 240 interrupted CTG repeats in exon 15 located

   Depositor

   Thomas Cooper

   Article

   Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   EGFP and human DMPK exons 11-15 with 240 interrupted CTG repeats (DMPK Human)
   

   Use

   Tetracycline responsive and bidirectional

   Expression

   Mammalian

   Promoter

   tetracycline responsive and bidirectional

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFV2-HA

   Plasmid

   #187082

   Purpose

   Expression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)

   Depositor

   Steven Vik

   Article

   Bae et al Biochim Biophys Acta. 2009 Sep;1787(9):1

   Insert

   atpBEFHAGDC
   

   Tags

   6-His beta subunit (atpD) and HA tag subunit a (a…

   Expression

   Bacterial

   Mutation

   All Cys codons changed to Ala.

   Available Since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AICSDP-45: TTN-mEGFP

   Plasmid

   #114412

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human TTN, via Cas9-excisable CAGGS-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

   Insert

   TTN Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TTN Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTipi2.1-AZU_SP

   Plasmid

   #229144

   Purpose

   Allows for expression of recombinant antibodies. Includes human azurocidin signal peptide.

   Depositor

   Institute for Protein Innovation (IPI)

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Jan. 9, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA_RFP-TEVprotease

   Plasmid

   #124947

   Purpose

   Expression of RFP-TEVprotease

   Depositor

   Matthew Good, Daniel Hammer, Benjamin Schuster

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RFP-TEVprotease
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBIG1b

    Plasmid

    #80612

    Purpose

    pBIG1b - Step1 vector b (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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