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pCIBN-tagBFP-TetR

Plasmid

#103797

Purpose

BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-TetR (CIB1, tetR Mustard Weed, E. coli)

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (1-90) (72P)

Plasmid

#62080

Purpose

Expression of human p53 (1-90) (72P) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

contains TAD/PP residues 1-90; 72P

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
Control-cx43.4-48H-pPRISM

Plasmid

#117801

Purpose

Control to test technique on a target that is known to work

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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mitoTALECD for_atp1_1397CN

Plasmid

#186203

Purpose

For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87

Depositor

Shin-ichi Arimura

Article

Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

Inserts

mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)

Use

Synthetic Biology; Ti plasmid for plant transform…

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSUMO CPTP

Plasmid

#170735

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

CPTP (CPTP Human)

Tags

SUMO

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CreLite

Plasmid

#131785

Purpose

AAV donor/transfer vector expressing CreLite system components, PhyBΔCreC and PIF6CreN, driven by CBh promoter

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC-P2A-PIF6CreN

Use

AAV, Cre/Lox, and Synthetic Biology

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR3-Nterm 6xHis mRFP-T500

Plasmid

#92317

Purpose

Expression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR3

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

Optimized NTerm 6xHis tagged monomeric RFP (mRFP)

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pFRT-DHX36iso2E335A

Plasmid

#159588

Purpose

Express N-ter FLAG HA tagged DHX36 isoform 2 with E335A mutation in mammalian cells

Depositor

Markus Hafner

Article

Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

Insert

DHX36 (DHX36 Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

E335A

Promoter

CMV

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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MKV937/nlp-1p::FlincG3

Plasmid

#140508

Purpose

This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

Depositor

Miri VanHoven

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

FlincG3

Expression

Worm

Promoter

nlp-1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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Clover-Mito-7

Plasmid

#56529

Purpose

Localization: Mitochondria, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Mito (COX8A Human)

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits