We narrowed to 4,185 results for: Gca

1. PX458_CEBPB_2

   Plasmid

   #64047

   Purpose

   Expresses gRNA against human CEBPB along with Cas9 with 2A GFP

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. ACE2 g3

   Plasmid

   #153013

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

   Depositor

   Jason Sheltzer

   Insert

   ACE2 (ACE2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

   Plasmid

   #118157

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-P2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. GESTALT_pX330-v1

   Plasmid

   #103061

   Purpose

   plasmid px330 with guide targeting GESTALT v1 through V5 constructs

   Depositor

   Jay Shendure

   Article

   McKenna et al Science. 2016 May 26. pii: aaf7907.

   Insert

   integration of Cas9 with guide targeting GESTALT barcodes V1 to V5
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJF131B

   Plasmid

   #113326

   Purpose

   pTet-W108 scRNA.b2, pTet-RR2-sgRNA

   Depositor

   Jesse Zalatan

   Article

   Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

   Insert

   W108 scRNA_MS2 and sgRNA_RR2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbS5c-AcrB/AcrD/AcrFa, ABO_0964

   Plasmid

   #86206

   Purpose

   Expresses efflux pump in E. coli to provide resistance to biofuel (low copy)

   Depositor

   Mary Dunlop

   Article

   Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

   Insert

   Efflux pump
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

   Available since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-Crispr-V2-HMID.v1

   Plasmid

   #103062

   Purpose

   LentiCrisprV2 with guide targeting V1-V5 GESTALT barcodes

   Depositor

   Jay Shendure

   Article

   McKenna et al Science. 2016 May 26. pii: aaf7907.

   Insert

   Cas9 plus expressed GESTALT V1-V5 guide
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-U6-RNF103-CHMP3

   Plasmid

   #140584

   Purpose

   Expresses a gRNA and mCherry in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   gRNA RNF103-CHMP3
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pColE1_sgRNA_pos7_AmpR

   Plasmid

   #119150

   Purpose

   Encodes sgRNA targeting position 7 in pColE1_70a_deGFP_KanR

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 7 in pColE1_70a_deGFP_KanR
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pColE1_sgRNA_pos5_AmpR

    Plasmid

    #119148

    Purpose

    Encodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 5 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. LRT2B-GO

    Plasmid

    #136908

    Purpose

    Lentivirus expressing GO sgRNA

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    sgGO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    hU6

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330.LoxP

    Plasmid

    #66586

    Purpose

    sgRNA for LSL

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    LoxP sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pLKO.1-blast-cfRab8b_3

    Plasmid

    #26709

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

    Insert

    dog Rab8b RNAi
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Dog Rab8b_3 RNAi.

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pFOXA3.1.0-gDNA

    Plasmid

    #112417

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor FOXA3

    Depositor

    Kevin White

    Insert

    FOXA3 (FOXA3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBSU6-shTOM40.3

    Plasmid

    #89796

    Purpose

    Expresses shRNA against TOM40.

    Depositor

    Robert Friedlander

    Article

    Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

    Insert

    shRNA Tom40
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330.iGFP3

    Plasmid

    #66583

    Purpose

    sgRNA for iGFP

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    iGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pQdCas12a.luxR(mut)-sggfp(B)

    Plasmid

    #236041

    Purpose

    The plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

    Depositor

    Radhakrishnan Mahadevan

    Insert

    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    quorum sensing promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCBE-dGFP-gRNA

    Plasmid

    #226862

    Purpose

    Human gRNA expression vector targeting the Y93H mutation of non-fluorescent EGFP in plasmid pCBE-dGFP; for use with CBEs

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    Sp-gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-U6-BRD4-HaloTag-Guide-hspCas9

    Plasmid

    #228576

    Purpose

    Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

    Depositor

    Marcin Imielinski

    Insert

    BRD4 gRNA (BRD4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti-ERCC1 gRNA2

    Plasmid

    #211631

    Purpose

    sgRNA-2 against ERCC1

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    sgRNA ERCC1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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