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DCT.VV.Orf46

Plasmid

#12734

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf46

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf47

Plasmid

#12735

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf47

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf49

Plasmid

#12737

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf49

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf54

Plasmid

#12742

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf54

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf57

Plasmid

#12745

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf57

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
Lentiviral-S-tdTomato

Plasmid

#112579

Purpose

Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

Depositor

Jeffrey Rothstein

Article

Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

Insert

NLS-tdTomato-NES

Use

Lentiviral

Tags

nuclear export sequence and nuclear localization …

Expression

Mammalian

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro

Plasmid

#205631

Purpose

Expression of TnpBmax in mammalian cells

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

ISDra2-TnpB optimized for mammalian expression

Use

Tags

NLS-3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

Plasmid

#124537

Purpose

Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

Depositor

Tudor Fulga

Article

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Insert

deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro

Use

Tags

Expression

Mammalian

Mutation

d11-41 C55G, d11-41 C55G

Promoter

Minimal ADE

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF1a-ETV2-IRES-Puro

Plasmid

#61061

Purpose

lentiviral vector for ETV2

Depositor

Igor Slukvin

Article

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

ETV2 (ETV2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1alpha

Available since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLX317-VRK1-K179E

Plasmid

#199644

Purpose

Expresses kinase-inactive VRK1 (K179E)

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK1 (VRK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

K179E

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCas9-sgVRK1 #4-Blast

Plasmid

#199647

Purpose

Expresses Cas9 and sgRNA guide targeting VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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BLADE

Plasmid

#134912

Purpose

Empty backbone for cloning sgRNA sequence to be used in nanoblades system

Depositor

Théophile Ohlmann, Emiliano Ricci, Philippe Mangeot

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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SPLICS NU-MT P2A

Plasmid

#210129

Purpose

Detect the Nucleus-Mitochondria contact

Depositor

Tito Calì

Article

Wu et al EMBO J. 2022 Dec 15;41(24):e112006. doi:

Insert

SPLICS NU-MT P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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PB-TET-mcMyc

Plasmid

#20888

Purpose

piggyBac vector with tet inducible mcMyc

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

cMyc (Myc Mouse)

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

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AAV CAMKII PSAM4 GlyR IRES EGFP

Plasmid

#119744

Purpose

Chemogenetic inhibitor expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Has Service

AAV5

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)

Use

AAV

Tags

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

CamKII

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-SPI1

Plasmid

#97039

Purpose

Expresses SPI1 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Spi-1 Proto-Oncogene (SPI1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Tet on

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-ArchT-GFP

Plasmid

#29777

Purpose

AAV-mediated expression of ArchT-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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AAV SYN flex PSAM4 GlyR IRES EGFP

Plasmid

#119741

Purpose

Cre-dependent chemogenetic inhibitor expression

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Has Service

AAV5 and AAV9

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)

Use

AAV

Tags

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

synapsin

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-Arch-GFP

Plasmid

#22222

Purpose

Cre-dependent viral expression of Arch-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Has Service

AAV1 and AAV9

Insert

Arch

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

no

Promoter

CAG

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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AAV-mPGK-eGFP (beta-globin intron)

Plasmid

#162513

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intron

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

Insert

eGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

mPGK

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge