We narrowed to 13,486 results for: cas9 genes

1. pHAGE EF1α dCas9-NED

   Plasmid

   #109369

   Purpose

   Lentiviral vector for expressing effector domains fused to dCas9.

   Depositor

   Antal Kiss

   Article

   Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-SpCas9-GFP-U6-gRNA

   Plasmid

   #79144

   Purpose

   All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

   Depositor

   Jizhong Zou

   Article

   Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. LLP185 pLVP-dCas9-DNMT3a V2

   Plasmid

   #100936

   Purpose

   dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and 3xTy1

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Icam 2 SaCas9 YAP sgRNA2

   Plasmid

   #99735

   Purpose

   Expresses SaCas9 and a gRNA targeting mouse YAP

   Depositor

   Yu Huang

   Article

   Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

   Insert

   SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDL17_Cas9-His Δcys M1C

   Plasmid

   #206251

   Purpose

   Bacterial expression of SpCas9 Δcys M1C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   M1C, C80S, C574S

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p221z-CAS9p-TagRFP-t35s

   Plasmid

   #118386

   Purpose

   Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Insert

   CAS9p-TagRFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-EF1alpha-dCas9-KRAB_Hygro

   Plasmid

   #192663

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, D839A, H840A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pScRad52-SpCas9 (pXK-1646)

   Plasmid

   #208823

   Purpose

   CRISPR/Rad52-Cas9 expression vector for Animal gene editing, harboring the ScRad52-SpCas9 and a VEGFA.gRNA expression cassette. Key Construct: hU6-VEGFa.gRNA-CMV-ScRad52-SpCas9.

   Depositor

   Kun Xu

   Article

   Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-

   Type

   Empty backbone

   Use

   CRISPR; Cas9

   Tags

   Rad52

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PET-WT SpCas9-NLS-6xHis

   Plasmid

   #207373

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   WT SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-TO-dCas9-P2A-HSA

    Plasmid

    #121936

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    dCas9-P2A-HSA
    

    Use

    CRISPR and Lentiviral

    Tags

    HSA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-Cas9-P2A-Puro

    Plasmid

    #110837

    Purpose

    Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

    Plasmid

    #222991

    Purpose

    Bacterial expression of iGeoCas9(C2) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
    

    Use

    CRISPR

    Tags

    CL7, His, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. LLP339 pGK-dCas9-SunTag

    Plasmid

    #100943

    Purpose

    dCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1a

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    dCas9, SunTag array (10xGCN4)
    

    Use

    CRISPR

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330GFP-hU6-gRNA-hSpCas9

    Plasmid

    #128385

    Purpose

    GFP positive gRNA/hSpCas9 construct

    Depositor

    Budd A. Tucker

    Article

    Giacalone et al Curr Protoc Stem Cell Biol. 2018 Feb 28;

    Insert

    GFP, gRNA and hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

    Plasmid

    #222993

    Purpose

    Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

    Depositor

    Jennifer Doudna

    Article

    Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

    Insert

    HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
    

    Use

    CRISPR

    Tags

    Puro

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid

    #71828

    Purpose

    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    BACH2-sgRNA8 (BACH2 Synthetic, Human, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    U6

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. HiFi-nCas9-NG_P2A-mCherry

    Plasmid

    #219936

    Purpose

    Mammalian expression of Sp-HiFi-nCas9-NG with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-HiFi-nCas9(D10A)-NG_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    HiFi-nCas9(D10A/R691A)

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX380 Treponema denticola Cas9

    Plasmid

    #68317

    Purpose

    Treponema denticola Cas9

    Depositor

    Feng Zhang

    Insert

    TdCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid

    #71685

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, Human, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3xFLAG-dCas9/pMXs-puro

    Plasmid

    #51240

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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