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pQCXIH-Myc-YAP-S94A

Plasmid

#33094

Depositor

Kunliang Guan

Publication

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

S94A

Promoter

CMV/MSV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles
pBbAW4k-loxP-TT-loxP-mRFP1

Plasmid

#134405

Purpose

Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

Depositor

Mary Dunlop

Publication

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

W4-loxP-TT-loxP-mRFP1

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

W4 (modified T7A1)

Available since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mTFP1-C1

Plasmid

#54613

Purpose

Localization: C1 Cloning Vector, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Publication

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mTFP1

Expression

Mammalian

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMXs-NKX6.1

Plasmid

#32934

Depositor

Kevin Eggan

Publication

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

NKX6.1 (NKX6-1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pLM494

Plasmid

#100539

Purpose

CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.

Depositor

Bernd Müller-Röber

Publication

Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:

Inserts

GGPP synthase
phytoene desaturase
phytoene-beta carotene synthase
HMG CoA reductase

Use

Cre/Lox and Synthetic Biology

Expression

Yeast

Promoter

PGK1, TDH3, TIP1, and ZEO1

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEGFP-C3-PLK4-3xFLAG

Plasmid

#69837

Purpose

This plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Publication

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pJJR82

Plasmid

#75027

Purpose

EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Publication

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

EGFP^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized EGFP

Expression

Worm

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

Plasmid

#207664

Purpose

Neuropeptide degradation

Depositor

Sung Han

Publication

Kim et al bioRxiv. 2023 Jan 20:2023.01.19.524797.

Insert

NEP (MME Human)

Use

AAV and Cre/Lox

Tags

mRUBY3

Mutation

Addition of signaling peptide (POMC) on the N-ter…

Promoter

hSynapsin

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Rabbit GFP Cav 2.2 pMT2

Plasmid

#58737

Purpose

expression of GFP tagged rabbit Cav2.2 in mammalian cells

Depositor

Annette Dolphin

Publication

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cav2.2 (CACNA1B Rabbit)

Tags

GFP

Expression

Mammalian

Promoter

SV40

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

Plasmid

#83925

Purpose

Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

Depositor

Charles Gersbach

Publication

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

eGFP
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Promoter

hUbC

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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GSK3betaS9A (1016)

Plasmid

#49492

Purpose

expresses human Gsk3beta with S9A (constitutively active) mutation

Depositor

Scott Friedman

Publication

Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GSK3beta S9A (GSK3B Human)

Expression

Mammalian

Mutation

S9A (constitutively active)

Promoter

CMV

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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His6-SpyCatcher-mi3

Plasmid

#113043

Purpose

Expresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

Depositor

Mark Howarth

Publication

Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

Insert

His6-SpyCatcher-mi3

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGWB514

Plasmid

#74856

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLX304_CMV::mCherry-LgBiT-FLAG-NES

Plasmid

#199715

Purpose

Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

Depositor

Alice Ting

Publication

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

mCherry-LgBiT-FLAG-NES

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-UbC

Plasmid

#62806

Purpose

To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

Depositor

Jae Lee, Pantelis Tsoulfas

Publication

Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

Type

Empty backbone

Use

AAV; Eucaryotic

Expression

Mammalian

Promoter

hUbC

Available since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_MMP-9-HA

Plasmid

#121172

Purpose

Expression of mouse MMP9

Depositor

M. Elizabeth Fini, Shinwu Jeong

Publication

Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

Insert

MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)

Tags

HA

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV immediate/early

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAG-HA-NLGN3 WT

Plasmid

#59318

Purpose

Expression plasmid of HA-tagged wildtype human Neuroligin 3

Depositor

Peter Scheiffele

Publication

Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

Insert

NLGN3 (NLGN3 Human)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDEST-FRT/T0-GFP-PALB2

Plasmid

#71113

Purpose

mammalian expession of GFP-PALB2

Depositor

Daniel Durocher

Publication

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PALB2 (PALB2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV/TO

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pcDNA3-hPar2

Plasmid

#53228

Purpose

mammalian expression of human Par2

Depositor

Shaun Coughlin

Publication

Bohm et al Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3)

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Par2 (F2RL1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBMN_HA-TBK1 (kinase dead - D135N)

Plasmid

#199206

Purpose

Used for the expression of TBK1 carrying a kinase dead mutation D135N (SMC Internal No. 1997)

Depositor

Sascha Martens

Publication

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

D135N

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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