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pLKO.1B CDC25C_1

Plasmid

#160769

Purpose

Suppress CDC25C

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCDC25C_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1B CDC25A_1

Plasmid

#160767

Purpose

Suppress CDC25A

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCDC25A_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314-U6-A34G/A35G/G96C/A97C/U100C/U101C

Plasmid

#81239

Purpose

U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U101C) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with A34G/A35G/G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-PIWIL2 ts2

Plasmid

#115870

Purpose

PIWIL2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

PIWIL2 shRNA (PIWIL2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN FKBP(DD)-YFP

Plasmid

#31763

Depositor

Thomas Wandless

Article

Banaszynski et al Cell. 2006 Sep 8;126(5):995-1004.

Insert

FKBP(DD)-YFP-HA (FKBP1A Human)

Use

Retroviral

Tags

HA and YFP

Mutation

F36V, L106P on FKBP12

Promoter

LTR

Available Since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pLEX-FLAG-NRAS-IRES-Puro

Plasmid

#120574

Purpose

Expresses Flag-N-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-NRASQ61K-IRES-Puro

Plasmid

#120570

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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Rat SERCA2b (pMT2)

Plasmid

#75184

Purpose

Mammalian expression of ATP2A2

Depositor

Jonathan Lytton, Gary Shull

Article

Gunteski-Hamblin et al J Biol Chem. 1988 Oct 15;263(29):15032-4

Insert

ATP2A2

Expression

Mammalian

Promoter

adenovirus major late promoter

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 N

Plasmid

#158079

Purpose

Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 nucleocapsid (N )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)

Plasmid

#165456

Purpose

Inducible ZEB2 expression from AAVS1 locus

Depositor

Madeline Lancaster

Article

Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

Insert

ZEB2 (ZEB2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-FLAG-NRASQ61K-IRES-Puro

Plasmid

#120575

Purpose

Expresses Flag-N-Ras Q61K fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS hygro

Plasmid

#158634

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS construct

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro

Plasmid

#120563

Purpose

Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 M

Plasmid

#158078

Purpose

Expresses SARS-CoV-2 membrane protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 matrix (M )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 E

Plasmid

#158080

Purpose

Expresses SARS-CoV-2 envelope protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 envelope (E )

Expression

Mammalian

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro shPOLE1_1

Plasmid

#160810

Purpose

Express Dox repressible shPOLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE1_1

Use

Lentiviral

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pPB9P2

Plasmid

#210030

Purpose

Contains PE2 prime editing gRNA cloning site, inducible prime editor 2, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

prime editor 2
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1CAN

Plasmid

#178032

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Promoter

T7LacI

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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MARS

Plasmid

#205232

Purpose

Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-i in mammalian cells

Depositor

Jeremy Baskin

Article

Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816

Insert

PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)

Tags

Nuclear Export Sequence and mScarlet-i

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli3/Flag

Plasmid

#51246

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only