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pBiex-1 GFPNanobody_SNAP

Plasmid

#82711

Purpose

Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

Depositor

Sivaraj Sivaramakrishnan

Publication

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

GFP Nanobody

Tags

6xHis Purification tag, FLAG purification tag, an…

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRRL_SRSF2_P95H_mCherry

Plasmid

#84023

Purpose

expression of SRSF2 mutant in mammalian cells

Depositor

Robert Bradley

Publication

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Mutation

P95H

Promoter

PGK

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

Plasmid

#67985

Purpose

Cas9 activity reporter (control) with mCherry and BFP

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherryABFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMT-Hrp48-ADARcd-V5

Plasmid

#81172

Purpose

identification of cell-specific targets of RNA binding proteins

Depositor

Michael Rosbash

Publication

McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

Insert

Hrp48-ADARcd fusion (Hrb27C Fly)

Expression

Insect

Promoter

Drosophila metallothionein promoter

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FUGW-eGFP-ZEB1 3'UTR

Plasmid

#81018

Purpose

FUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscript

Depositor

Jeffrey Rosen

Publication

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

Zeb1 3'UTR (UTS2R Human)

Use

Lentiviral

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pINDUCER13 miR-200c-141

Plasmid

#81020

Purpose

Lentiviral vector containing doxycycline inducible human miR-200c. Puromycin selectable.

Depositor

Jeffrey Rosen, Thomas Westbrook

Publication

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

miR-200c (MIR200C Human)

Use

Lentiviral

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Actin-cpstFRET-Actin

Plasmid

#80643

Purpose

cpstFRET actin sensor for mechanical force measurements in cytoskeleton

Depositor

Fred Sachs

Publication

Guo et al Proc Natl Acad Sci U S A. 2014 Dec 9;111

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Beta actin, cpstFRET

Expression

Mammalian

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pKA-141

Plasmid

#79832

Purpose

Expresses RpBphP1-mCherry

Depositor

Vladislav Verkhusha

Publication

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BphP1-mCherry

Expression

Mammalian

Promoter

CMVd1

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLgw EcoDam-V5-RFC1

Plasmid

#59209

Purpose

Mammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloning

Depositor

Bas van Steensel

Publication

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Lentiviral; Damid

Tags

Dam and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mCherry-Rab9a

Plasmid

#78592

Purpose

Express mCherry-Rab9a in mammalian cells

Depositor

Yihong Ye

Publication

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab9a (RAB9A Human)

Tags

mCherry2

Expression

Mammalian

Promoter

CMV

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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