164,765 results

1. pCAG-ASAP3ER

   Plasmid

   #195356

   Purpose

   ASAP3ER under the control of the pCAG promoter

   Depositor

   Brenda Bloodgood

   Article

   Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

   Insert

   ASAP3ER
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. JC Mad-1

   Plasmid

   #25626

   Depositor

   Peter Howley

   Article

   Howley et al J Virol. 1980 Dec . 36(3):878-82.

   Insert

   JC Virus Mad-1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

3. H2B-2A-GFP-IRESpuro2

   Plasmid

   #183746

   Purpose

   Expresses H2B-2A-GFP

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   H2B (H2BC16P Human)
   

   Use

   Tags

   GFP with 2A protease cleavage site between H2B an…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. HIP-flash (HOP-flash mutant)

   Plasmid

   #83466

   Purpose

   Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene

   Depositor

   Barry Gumbiner

   Article

   Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

   Insert

   Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMito-TrxRFP1

   Plasmid

   #98997

   Purpose

   mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized

   Depositor

   Huiwang Ai

   Article

   Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

   Insert

   TrxRFP1 and mitochondrial localization sequence
   

   Use

   Tags

   His-tag and Mitochondria localization sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBiFC-VN173

   Plasmid

   #22010

   Purpose

   Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   VN173
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Venus residus 1-172

   Promoter

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX-sgRNA

   Plasmid

   #50662

   Purpose

   Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   sgAAVS1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-TRKB-V5/HIS

   Plasmid

   #205614

   Purpose

   Expression of human TRKB receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   TRKB receptor tyrosine kinase (NTRK2 Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAB076i6

   Plasmid

   #79200

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (no operator) gIII, luxAB; Ppro1 434cI-SH2ABL1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (no operator); Ppro1

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti_CamKIIa_GFP

    Plasmid

    #96941

    Purpose

    To label mature excitatory iPSC-derived human neurons

    Depositor

    Ricardo Dolmetsch, Oleksandr Shcheglovitov

    Article

    Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

    Insert

    Green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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