164,799 results

1. pEMS1602

   Plasmid

   #29305

   Purpose

   Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).

   Depositor

   Elizabeth Simpson

   Article

   Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

   Insert

   Ple131 (MKI67 Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Intron-LacZ-NLS

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 4, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. miR-124-5 promoter GFP

   Plasmid

   #20792

   Depositor

   David Bartel

   Article

   Shkumatava et al Genes Dev. 2009 Feb 15. 23(4):466-81.

   Insert

   mir-124 (mir124-5 Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available Since

   May 11, 2009

   Availability

   Academic Institutions and Nonprofits only

3. PET30B-PTEN

   Plasmid

   #20741

   Depositor

   Alonzo Ross

   Article

   Redfern et al Biochemistry. 2008 Feb 19. 47(7):2162-71

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 2, 2009

   Availability

   Academic Institutions and Nonprofits only

4. Calb1-T2A-dgCre targeting vector

   Plasmid

   #114438

   Purpose

   Target dgCre into the endogenous mouse Calb1 locus at the stop codon

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   dgCre
   

   Use

   Mouse Targeting

   Tags

   DHFR domain and EGFP

   Expression

   Mutation

   Promoter

   none; utilizes endogenous Calb1 promoter for expr…

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGal4BD-CRY2

   Plasmid

   #28243

   Purpose

   Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   Gal4BD

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCVhyg-DEST

   Plasmid

   #119747

   Purpose

   Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Hygromycin resistance)

   Depositor

   Martin Reijns, Andrew Jackson

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti_EF1a_Flag_EGFP

   Plasmid

   #183117

   Purpose

   Expresses flag-tagged EGFP in mammalian cells

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-GRAB-rDA2h

   Plasmid

   #208701

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h in neurons

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA2h
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3. Flag-CSL WT

   Plasmid

   #167004

   Purpose

   Mammalian expression of CSL WT

   Depositor

   Gian-Paolo Dotto

   Article

   Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

   Insert

   CSL (RBPJ Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9(BB)-2A-Puro (PX459)

    Plasmid

    #48139

    Purpose

    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only