We narrowed to 13,017 results for: SHI

1. pCS2plus-mVenus1-155-I152L-GGGS-TDP-43

   Plasmid

   #162614

   Purpose

   Allows for transcription of improved mVenus I152L fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

   Depositor

   Marco Morsch

   Article

   Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97308

   Purpose

   HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HMEJ donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-M-Flag-BAP1-H169Q

   Plasmid

   #125847

   Purpose

   express mutant BAP1-H169Q

   Depositor

   Boyi Gan

   Article

   Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

   Insert

   BAP1 (BRCA1 associated protein 1) (BAP1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   H169Q

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97311

   Purpose

   MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb MMEJ donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

   Plasmid

   #197024

   Purpose

   Vector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized OsCas12f1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh, CMV, hU6

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEN6-Ubc5 Sc

   Plasmid

   #83234

   Purpose

   pHis-par2-UbHs/Ubc5Sc/ Uba1Ta

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Inserts

   Ubiqutin (UBC Human)
   Ubc 5 (UBC5 Budding Yeast)
   Uba 1
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. WP_010880027.1

   Plasmid

   #194729

   Purpose

   Lumazine synthase of the hyperthermophile Aquifex aeolicus bacterium

   Depositor

   Fang Li

   Article

   Geng et al PLoS Pathog. 2021 Sep 7;17(9):e1009897.

   Insert

   nanoparticle-proteinA and His tag
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP

   Plasmid

   #75123

   Purpose

   Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tag

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Tags

   3x FLAG-tag

   Expression

   Mutation

   bromodomain replaced with the first bromodomain o…

   Promoter

   Available since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-dCas12a-A3A-Y132D

   Plasmid

   #193641

   Purpose

   Expression of base editor dCas12a-A3A-Y132D in mammalian cells for base editing

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3A-Y132D
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A-Y132D

   Promoter

   CMV

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-dCas12a-A3B

    Plasmid

    #193642

    Purpose

    Expression of base editor dCas12a-A3B in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-hA3B, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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