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pGEMHE:hTRAAK(Q258K)

Plasmid

#133077

Purpose

X. laevis expression vector. It will generate the human TRAAK channel

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)

Mutation

Q258K

Promoter

T7

Available Since

Oct. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2214 - Intron GG1 - 900 bp

Plasmid

#159880

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - 900 bp

Expression

Worm

Mutation

Not applicable

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1359 - Intron GG2 - smu-1

Plasmid

#159805

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - smu-1

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGFPCPB1

Plasmid

#23009

Depositor

Linda Wordeman

Article

Wordeman et al J Cell Biol. 2007 Dec 3. 179(5):869-79.

Insert

EGFP-CenpB

Tags

EGFP

Expression

Mammalian

Mutation

deleted CenpB amino acids 169-606, T145S

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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HLCS_pCSdest

Plasmid

#53803

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

HLCS (HLCS Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pMpGWB330

Plasmid

#68658

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

tdTomato

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA-NC1

Plasmid

#248557

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA-NC1 TD#277

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCFJ1117 - [4-1] - Promoter - Psmu-1(no_atg)

Plasmid

#159886

Purpose

Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[4-1] - Promoter - Psmu-1(no_atg)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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PCBP3_pCSdest

Plasmid

#53957

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PCBP3 (PCBP3 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
IGSF5_pCSdest

Plasmid

#53886

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

IGSF5 (IGSF5 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
WRB_pCSdest

Plasmid

#53856

Purpose

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

WRB (GET1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pMpGWB214

Plasmid

#68605

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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PDE9A_pCSdest

Plasmid

#53944

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PDE9A (PDE9A Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBT255_(pCA-GFP4m)

Plasmid

#36877

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

GFP

Expression

Mammalian

Mutation

F64L; S65T; V163A; S175G

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pDOC-K-glmS-lacIZ

Plasmid

#158061

Purpose

Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome

Depositor

Mark Webber

Article

Holden et al Microbiology (Reading). 2020 Nov 23. doi

Insert

lacIZ operon

Use

Synthetic Biology

Expression

Bacterial

Promoter

lac

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMX240

Plasmid

#23010

Depositor

Linda Wordeman

Article

Wordeman et al J Cell Biol. 2007 Dec 3. 179(5):869-79.

Insert

GCPBM

Tags

CenpB 1-167 and EGFP

Expression

Mammalian

Mutation

deleted MCAK amino acids 1-178, 584-718 mutated …

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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KCNJ15_pCSdest

Plasmid

#53840

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

KCNJ15 (KCNJ15 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pDGE7

Plasmid

#79462

Purpose

sgRNA shuttle vector; module 2

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE9

Plasmid

#79465

Purpose

sgRNA shuttle vector; module 3

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE11

Plasmid

#79467

Purpose

sgRNA shuttle vector; module 4E

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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