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We narrowed to 2,405 results for: dcas9

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Showing: 1741 - 1760 of 2405 results

  3. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

    Plasmid
    #208944
    Purpose
    A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208944-plasmid-map-sequence-id-415672

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  5. pMOD6-pGRR-r11-r10-yeGFP

    Plasmid
    #74368
    Purpose
    pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.
    Depositor
    Eric Klavins
    Publication
    Gander et al bioRxiv 041871
    Insert
    yeGFP
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    pGRR-r11-r10
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74368-plasmid-map-sequence-id-141319

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  6. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139091
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139091-plasmid-map-sequence-id-273888

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  7. pGGE-AtHDA6

    Plasmid
    #246810
    Purpose
    A GreenGate entry vector containing coding region of AtHDA6
    Depositor
    Danhua Jiang
    Insert
    AtHDA6 (HDA6 Mustard Weed)
    Use
    Greengate cloning entry vector
    Available since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246810-plasmid-map-sequence-id-494329

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  8. pGGE-AtREF6

    Plasmid
    #246813
    Purpose
    A GreenGate entry vector containing coding region of AtREF6
    Depositor
    Danhua Jiang
    Insert
    AtREF6
    Use
    Greengate cloning entry vector
    Available since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246813-plasmid-map-sequence-id-494308

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  9. pGGC-AtUBQ10 promoter-Insulator

    Plasmid
    #246799
    Purpose
    A GreenGate entry vector containing a AtUBQ10 promoter and an Insulator
    Depositor
    Danhua Jiang
    Insert
    AtUBQ10 promoter-Insulator
    Use
    Greengate cloning entry vector
    Available since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246799-plasmid-map-sequence-id-494302

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  10. pGGE-AtFIE

    Plasmid
    #246808
    Purpose
    A GreenGate entry vector containing coding region of AtFIE
    Depositor
    Danhua Jiang
    Insert
    AtFIE (FIE Mustard Weed)
    Use
    Greengate cloning entry vector
    Available since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246808-plasmid-map-sequence-id-494301

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  11. pGGE-AtJMJ14

    Plasmid
    #246811
    Purpose
    A GreenGate entry vector containing coding region of AtJMJ14
    Depositor
    Danhua Jiang
    Insert
    AtJMJ14
    Use
    Greengate cloning entry vector
    Available since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246811-plasmid-map-sequence-id-494297

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  12. pGGE-AtLDL1

    Plasmid
    #246812
    Purpose
    A GreenGate entry vector containing coding region of AtLDL1
    Depositor
    Danhua Jiang
    Insert
    AtLDL1 (LDL1 Mustard Weed)
    Use
    Greengate cloning entry vector
    Available since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246812-plasmid-map-sequence-id-494294

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  13. pGGC-ZmUBI Promoter-Insulator

    Plasmid
    #246800
    Purpose
    A GreenGate entry vector containing a ZmUBI promoter and an Insulator
    Depositor
    Danhua Jiang
    Insert
    ZmUBI Promoter-Insulator
    Use
    Greengate cloning entry vector
    Available since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246800-plasmid-map-sequence-id-494255

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  17. KK701: pMAGIC (L3-L2) 3x HA eptitope tag + polyA; hU6::xCas9(3.7) gRNA scaffold

    Plasmid
    #121842
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven xCas9(3.7) gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA fusion to dCas9 w/ gRNA expression
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121842-plasmid-map-sequence-id-237396

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  18. pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid
    #234370
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs
    Depositor
    Michael Smanski
    Publication
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Inserts
    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-13XGP41
    gRNA 1-1 and gRNA 1-2
    Use
    Synthetic Biology
    Tags
    GB1, GP41, and sfGFP
    Expression
    Plant
    Promoter
    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234370-plasmid-map-sequence-id-469430

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  19. pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid
    #234372
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs
    Depositor
    Michael Smanski
    Publication
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Inserts
    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-24XGP41
    gRNA 1-1 and gRNA 1-2
    Use
    Synthetic Biology
    Tags
    GB1, GP41, and sfGFP
    Expression
    Plant
    Promoter
    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234372-plasmid-map-sequence-id-469423

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Showing: 1741 - 1760 of 2405 results