We narrowed to 3,800 results for: golden gate

1. DVC_pBR322_EF

   Plasmid

   #217599

   Purpose

   Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. DVC_pBR322_FG

   Plasmid

   #217600

   Purpose

   Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DVC_pBR322_GH

   Plasmid

   #217601

   Purpose

   Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. DVC_pBR322_AE

   Plasmid

   #217597

   Purpose

   Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. DVC_p15A_GH

   Plasmid

   #217591

   Purpose

   Destination vector with chloramphenicol resistance and p15A origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. DVA_pBR322_AF

   Plasmid

   #217608

   Purpose

   Destination vector with ampicillin resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 0 or 2 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. DVA_pBR322_EH

   Plasmid

   #217609

   Purpose

   Destination vector with ampicillin resistance and pBR322 origin of replication. Carries E-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBH502

   Plasmid

   #215781

   Purpose

   NBU2 backbone with LacI(YQR) and P(O1)_sgRNA targeting Nanoluc

   Depositor

   Corey Wilson

   Article

   Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

   Inserts

   LacI(YQR)
   sgRNA-ribozyme cassette
   Nanoluc
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Bt1311, P(O1), and cfxA

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET22b-Empty-cleavableELP

   Plasmid

   #219401

   Purpose

   Insert protease recognition site with BsaI to make custom protease-responsive elastin-like polypeptide (ELP)

   Depositor

   Danielle Mai

   Article

   Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

   Insert

   Empty-cleavableELP
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET22b-FXa-cleavableELP

    Plasmid

    #219403

    Purpose

    Factor Xa-responsive elastin-like polypeptide (ELP)

    Depositor

    Danielle Mai

    Article

    Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

    Insert

    FXa-cleavableELP
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET22b-EK-cleavableELP

    Plasmid

    #219404

    Purpose

    Enterokinase-responsive elastin-like polypeptide (ELP)

    Depositor

    Danielle Mai

    Article

    Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

    Insert

    EK-cleavableELP
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET22b-Thr-cleavableELP

    Plasmid

    #219405

    Purpose

    Thrombin-responsive elastin-like polypeptide (ELP)

    Depositor

    Danielle Mai

    Article

    Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

    Insert

    Thr-cleavableELP
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBTK1059

    Plasmid

    #209549

    Purpose

    BTK Type 5 homology arm for Golden Gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Lariviere et al bioRxiv 2023.09.19.558440

    Insert

    Downstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
    

    Use

    Synthetic Biology

    Available Since

    Feb. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. GGDestX2-Amp

    Plasmid

    #157654

    Purpose

    Golden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNA

    Depositor

    Karmella Haynes

    Article

    Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6, CBh

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQ131C-RZ-Lb

    Plasmid

    #134347

    Purpose

    Golden Gate entry vector; empty vector to clone the 1st LbCas12a crRNA under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Li et al Plant Biotechnol J. 2019 Nov 15. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pICSL12022

    Plasmid

    #117510

    Purpose

    AtAG promoter Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:GGAG:pAG:AATG:BsaI
    

    Expression

    Plant

    Promoter

    AtAG

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJet1.2 'rncS

    Plasmid

    #117129

    Purpose

    smc gene upstream homology region

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Insert

    'rncS
    

    Use

    Golden gate donor vector for gene targeting in ba…

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGGE003

    Plasmid

    #48854

    Purpose

    Provides the At1g04880 terminator as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    At1g04880 terminator
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGGC014

    Plasmid

    #48827

    Purpose

    Provides GFP as GreenGate coding sequence module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    GFP
    

    Use

    Golden gate compatible cloning vector

    Mutation

    A206K

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGGC024

    Plasmid

    #48829

    Purpose

    Provides BFP as GreenGate coding sequence module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    BFP
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge