We narrowed to 1,753 results for: nif

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pET_His6-SUMO-TEV-TtCsm6

Plasmid

#172486

Purpose

Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

T. thermophilus Csm6 (codon optimized) (csm6 Synthetic)

Tags

His-SUMO-TEV

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mScarlet-STIM2 WPRE

Plasmid

#236236

Purpose

AAV expression of human STIM2 internally tagged with mScarlet-I

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mScarletI-STIM2 (STIM2 Human)

Use

AAV

Tags

mScarletI in position 29

Mutation

Signal peptide from STIM1 and residues 15-746 of …

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-CRY2oligo-mCherry-ANXA11(186-505 aa)

Plasmid

#164208

Purpose

Expresses CRY2oligo-mcherry tagged ANXA11 C terminal under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Tags

CRY2oligo-mcherry

Expression

Mammalian

Mutation

186-505 aa

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-CRY2oligo-mcherry-ANXA11(1-185aa)

Plasmid

#164207

Purpose

Expresses CRY2oligo-mcherry tagged ANXA11 N terminal under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Tags

CRY2oligo-mcherry

Expression

Mammalian

Mutation

1-185 aa

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald D40G

Plasmid

#164211

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Mutation

D40G

Promoter

EF1a

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBFC1207

Plasmid

#186694

Purpose

VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_2xBsaI_NT (Guide Stuffer) crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo
SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald R346C

Plasmid

#164213

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R346C mutant under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Mutation

R346C

Promoter

EF1a

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald R235Q

Plasmid

#164212

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R235Q mutant under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Mutation

R235Q

Promoter

EF1a

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-CRY2oligo-mcherry-ANXA11(D40G)

Plasmid

#164204

Purpose

Expresses CRY2oligo-mcherry tagged ANXA11 D40G mutant under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Tags

CRY2oligo-mcherry

Expression

Mammalian

Mutation

D40G

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES2-smGFP-Kv2.1

Plasmid

#131709

Purpose

Expresses Kv2.1 in mammalian cells

Depositor

Jennifer Kearney

Article

Kang et al Ann Neurol. 2019 Dec;86(6):899-912. doi:

Insert

KCNB1 (KCNB1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-CRY2oligo-mcherry-ANXA11(R235Q)

Plasmid

#164205

Purpose

Expresses CRY2oligo-mcherry tagged ANXA11 R235R mutant under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Tags

CRY2oligo-mcherry

Expression

Mammalian

Mutation

R235Q

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-CRY2oligo-mcherry-ANXA11(R346C)

Plasmid

#164206

Purpose

Expresses CRY2oligo-mcherry tagged ANXA11 R346C mutant under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Tags

CRY2oligo-mcherry

Expression

Mammalian

Mutation

R346C

Promoter

CMV

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON HaloTag-STIM2 WPRE

Plasmid

#236235

Purpose

AAV expression of human STIM2 internally tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

HaloTag-STIM2 (STIM2 Human)

Use

AAV

Tags

HaloTag in position 29

Mutation

Signal peptide from STIM1 and residues 15-746 of …

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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