We narrowed to 7,125 results for: trac

1. pX459-SpCas9-NG

   Plasmid

   #171370

   Purpose

   SpCas9-NG-mediated genome editing.

   Depositor

   Masahito Ikawa

   Article

   Oura et al Commun Biol. 2021 Jun 23;4(1):771. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for sgRNA; CBh for Cas9-2A-puroR

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBJ7

   Plasmid

   #167136

   Purpose

   A tetracyclin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Kanamycin promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-TRE3G-NLS-tdMCP-mCherry

   Plasmid

   #99909

   Purpose

   For the expression of NLS-tdMCP-mCherry

   Depositor

   Yujie Sun

   Article

   Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

   Insert

   NLS-tdMCP-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. mNeonGreen-IETD-NanoLuc

   Plasmid

   #98288

   Purpose

   caspase-8 BRET Sensor

   Depositor

   Maarten Merkx

   Article

   den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

   Insert

   mNeonGreen-LEHD-NanoLuc
   

   Use

   Tags

   His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.TBG mCherry-LDTS-LIRmut

   Plasmid

   #189003

   Purpose

   lipophagy-inducing adaptor protein, AAV, negative control

   Depositor

   Atsushi Hoshino

   Article

   Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

   Insert

   LDTS-LIRmut, mCherry
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-Dlx-tTA

   Plasmid

   #210730

   Purpose

   AAV vector expressing tetracycline-controlled transactivator (tTA) under Dlx promoter (for expressing tTA in GABAergic neurons)

   Depositor

   Wei Xu

   Article

   Thompson et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   tetracycline-controlled transactivator (tTA)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Dlx

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pN-PITCh-3XHA

   Plasmid

   #127881

   Purpose

   Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA tagging

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Insert

   GFP-Puro-3XHA (PPP2CA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-TRE3G-NLS-tdPCP-EGFP

   Plasmid

   #99910

   Purpose

   For the expression of NLS-tPMCP-EGFP

   Depositor

   Yujie Sun

   Article

   Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

   Insert

   NLS-tdPCP-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE4G

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPI138

   Plasmid

   #113229

   Purpose

   extrachromosomal expression vector, expression of LifeAct-GFP

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Insert

   LifeAct
   

   Use

   Dictyostelium discoideum expression vector, extra…

   Tags

   GFP

   Expression

   Mutation

   Promoter

   act15

   Available since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBluescriptSKII+ U6-sgRNA(F+E) ACTB

    Plasmid

    #74705

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNES-VL12.3

    Plasmid

    #43862

    Depositor

    Dane Wittrup

    Article

    Colby et al Proc Natl Acad Sci U S A. 2004 Dec 21;10

    Insert

    VL12.3
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pTCAV-FLEx(loxP)-FlpO

    Plasmid

    #67829

    Purpose

    Cre-dependent expression of FlpO from CAV2 virus

    Depositor

    Liqun Luo

    Article

    Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

    Insert

    FlpO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPy-CAG-Cre::ERT2-IRES-BSD

    Plasmid

    #48760

    Purpose

    CAG promoter driving Cre-ERT2 fusion-IRES-Blasticidin resistance

    Depositor

    Austin Smith

    Article

    Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

    Insert

    Cre::ERT2
    

    Use

    Ppy

    Tags

    ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME-PLIN2-tdTOMATO

    Plasmid

    #172533

    Purpose

    PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

    Depositor

    Richard White

    Article

    Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

    Insert

    plin2-tdtomato
    

    Use

    Tags

    Expression

    Mutation

    S80R, A81S, E144D, I148F, and R415H mutations in …

    Promoter

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 5HT2A_NB2_1

    Plasmid

    #127690

    Purpose

    Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    5HT2A_NB2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. CRY2low-mCherry

    Plasmid

    #104065

    Purpose

    Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherry

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CRY2low-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    CRY2 aa 1-491 truncation, R489E A491D

    Promoter

    CMV

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21b-LaG9(S151C)

    Plasmid

    #172490

    Purpose

    Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    LaG9(S151C)
    

    Use

    Tags

    6xHis, PPX cleavage site, and pelB leader sequenc…

    Expression

    Bacterial

    Mutation

    S151C

    Promoter

    T7

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTREX GPR68_NB2_2

    Plasmid

    #127691

    Purpose

    Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    GPR68_NB2_2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPBpuro-COX2-EBFP2

    Plasmid

    #184720

    Purpose

    Exogenous COX2

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    COX2-EBFP2 (PTGS2 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.1(+)-hH3-(GGS)X6-HaloTag C58

    Plasmid

    #171432

    Purpose

    Expression of hH3-(GGS)X6-HaloTag C58 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-hH3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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