Addgene

We narrowed to 38,161 results for: ANT;

Showing: 1761 - 1780 of 38161 results

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pGL3-CMV-Cdc42 3'UTR mutant (position 577-599)

Plasmid

#51391

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

NM_001039802.1 (CDC42 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mutation

miR-29 binding site mutant (position 577-599)

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
-962 human cyclin D1 promoter TCF (1)-(3) sites mutant pGL3Basic

Plasmid

#32735

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 Promoter (CCND1 Human)

Use

Luciferase

Tags

Expression

Mutation

-75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

Promoter

-962 CCND1

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

Plasmid

#101235

Purpose

Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant F491A/M495A/T496A/N497A/Q926A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

F491A, M495A, T496A, N497A and Q926A

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

Plasmid

#101234

Purpose

Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant T657A/G658A/W659A/R661A/Q926A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

T657A, G658A, W659A, R661A and Q926A

Promoter

T7

Available since

Nov. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

Plasmid

#101220

Purpose

Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant N692A/M694A/Q695A/H698A/Q926A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

N692A, M694A, Q695A, H698A and Q926A

Promoter

T7

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

Plasmid

#101232

Purpose

Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

G528A, V583A, E584A, D585A, N588A and Q926A

Promoter

T7

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA)

Plasmid

#229742

Purpose

Expresses Halo tagged human LRRK2 with LIR1 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

LIR1 mutant WEVL -> AEVA

Promoter

EF1A

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI -> ATFA)

Plasmid

#229743

Purpose

Expresses Halo tagged human LRRK2 with LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

LIR2 mutant WTFI -> ATFA

Promoter

EF1A

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-Cavbeta1 Ca2+ channel [N7/18R-rat IgG2a] - Chimeric

Plasmid

#231836

Purpose

Mammalian expression plasmid of anti-Cavbeta1 Ca2+ channel (Human) rat IgG2a R-mab. Derived from hybridoma N7/18.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Cavbeta1 Ca2+ channel (Homo sapiens) recombinant mouse monoclonal antibody (CACNB1 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-Iduna/RNF146 [N201/35R-rat IgG2a] - Chimeric

Plasmid

#231832

Purpose

Mammalian expression plasmid of anti-Iduna/RNF146 (Mouse) rat IgG2a R-mab. Derived from hybridoma N201/35.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Iduna/RNF146 (Mus musculus) recombinant mouse monoclonal antibody (Rnf146 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-KChIP3 K+ channel [K66/38R-rat IgG2a] - Chimeric

Plasmid

#231831

Purpose

Mammalian expression plasmid of anti-KChIP3 K+ channel (Rat) rat IgG2a R-mab. Derived from hybridoma K66/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-KChIP3 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnip3 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-FGF14/FHF4 [N56/21R-rat IgG2a] - Chimeric

Plasmid

#231830

Purpose

Mammalian expression plasmid of anti-FGF14/FHF4 (Mouse) rat IgG2a R-mab. Derived from hybridoma N56/21.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-FGF14/FHF4 (Mus musculus) recombinant mouse monoclonal antibody (Fgf14 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Kv3.4 K+ channel [N72/16R-rat IgG2a] - Chimeric

Plasmid

#231827

Purpose

Mammalian expression plasmid of anti-Kv3.4 K+ channel (Rat) rat IgG2a R-mab. Derived from hybridoma N72/16.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Kv3.4 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnc4 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Kv2.1 K+ channel [K39/25R-rat IgG2a] - Chimeric

Plasmid

#231822

Purpose

Mammalian expression plasmid of anti-Kv2.1 K+ channel (Human) rat IgG2a R-mab. Derived from hybridoma K39/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Kv2.1 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (Kcnb1 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Kv7.4/KCNQ4 K+ channel [N43/6R-rat IgG2a] - Chimeric

Plasmid

#231826

Purpose

Mammalian expression plasmid of anti-Kv7.4/KCNQ4 K+ channel (Human) rat IgG2a R-mab. Derived from hybridoma N43/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Kv7.4/KCNQ4 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (KCNQ4 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-SK2 K+ channel [K78/29R-rat IgG2a] - Chimeric

Plasmid

#231846

Purpose

Mammalian expression plasmid of anti-SK2 K+ channel (Rat) rat IgG2a R-mab. Derived from hybridoma K78/29.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-SK2 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnn2 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Kir2.3 K+ channel [N25/35R-rat IgG2a] - Chimeric

Plasmid

#231848

Purpose

Mammalian expression plasmid of anti-Kir2.3 K+ channel (Human) rat IgG2a R-mab. Derived from hybridoma N25/35.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Kir2.3 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (KCNJ4 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-GluN2C/NR2C [N422/18R-rat IgG2a] - Chimeric

Plasmid

#231849

Purpose

Mammalian expression plasmid of anti-GluN2C/NR2C glutamate receptor (Rat) rat IgG2a R-mab. Derived from hybridoma N422/18.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GluN2C/NR2C glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody (Grin2c Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Cavbeta4 Ca2+ channel [N10/7R-rat IgG2a] - Chimeric

Plasmid

#231825

Purpose

Mammalian expression plasmid of anti-Cavbeta4 Ca2+ channel (Rat) rat IgG2a R-mab. Derived from hybridoma N10/7.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Cavbeta4 Ca2+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Cacnb4 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Beta4-spectrin [N393/76R-rat IgG2a] - Chimeric

Plasmid

#231820

Purpose

Mammalian expression plasmid of anti-Beta4-spectrin (Human) rat IgG2a R-mab. Derived from hybridoma N393/76.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Beta4-spectrin (Homo sapiens) recombinant mouse monoclonal antibody (SPTBN4 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge