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Showing: 3521 - 3560 of 8321 results

  1. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Luciferase_shRNAmir

    Plasmid
    #178045
    Purpose
    CAG tdGFP Luciferase shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Luciferase_1309 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178045-plasmid-map-sequence-id-352919

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  2. pSBbi-RB-PE2

    Plasmid
    #173902
    Purpose
    SB-transposon with constitutive expression of SpCas9 PE2
    Depositor
    Ronald Cohn
    Insert
    PE2
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    173902-plasmid-map-sequence-id-346342

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  3. pKSB- attP 3xP3-GFPsv40 GGGG AAGA

    Plasmid
    #174534
    Purpose
    attP site and 3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Eric Marois
    Insert
    attP and 3xP3-GFP-SV40 BsaI cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    3xP3
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    174534-plasmid-map-sequence-id-343228

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  4. pKSB- PUb-GFPsv40 GGGG AAGA

    Plasmid
    #174536
    Purpose
    PUb-GFP fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Eric Marois
    Insert
    PUb-GFP-SV40 BsaI cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Aedes aegypti Polyubiquitin
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    174536-plasmid-map-sequence-id-343240

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  5. pKSB- PUb-GFPTub56D GGGG AAGA

    Plasmid
    #174537
    Purpose
    PUb-GFP fluorescence marker cassette for gene knock-in in mosquitoes, with DmTub56D terminator
    Depositor
    Eric Marois
    Insert
    PUb-GFP-Tub56Dterminator BsaI cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Aedes aegypti Polyubiquitin
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    174537-plasmid-map-sequence-id-343241

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  6. pISV-DsRed1-nopP

    Plasmid
    #171025
    Purpose
    Agrobacterium rhizogenes-mediated hairy root transformation of Lotus japonicus with nopP, a type 3 effector gene of Sinorhizobium sp. NGR234.
    Depositor
    Christian Staehelin
    Insert
    nopP
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2 X EN 35S
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    171025-plasmid-map-sequence-id-337715

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  7. pANY4-pL18-ccdB

    Plasmid
    #164898
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164898-plasmid-map-sequence-id-327989

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  8. PB CAG-sfGFP-GSAx9-iCre-ERT2

    Plasmid
    #137857
    Purpose
    CAG-sfGFP-GSAx9-iCre-ERT2 piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    sfGFP-GSAx9-iCre-ERT2
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    137857-plasmid-map-sequence-id-316181

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  9. rox Neo* rox

    Plasmid
    #134322
    Purpose
    rox-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Mouse Targeting; Dre/rox
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    134322-plasmid-map-sequence-id-316179

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  10. pXZ13lacZalphaKanR

    Plasmid
    #160230
    Purpose
    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    160230-plasmid-map-sequence-id-314765

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  11. pCRII-TOPO CMV-cGFP-SV40 pA + mMALAT1_3'

    Plasmid
    #91803
    Purpose
    Expresses a cGFP mRNA that ends in the MALAT1 triple helix when the upstream SV40 poly(A) signal fails to be used
    Depositor
    Jeremy Wilusz
    Insert
    Coral Green Fluorescent Protein (cGFP)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    91803-plasmid-map-sequence-id-180321

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  12. pX335 Mouse 5' Srcap gRNA B

    Plasmid
    #127905
    Purpose
    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene
    Depositor
    Mark Groudine
    Insert
    Srcap gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127905-plasmid-map-sequence-id-249688

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  13. pX335 Mouse 5' Srcap gRNA A

    Plasmid
    #127904
    Purpose
    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene
    Depositor
    Mark Groudine
    Insert
    Srcap gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127904-plasmid-map-sequence-id-249683

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  14. pX330 Mouse 3' Ubf gRNA

    Plasmid
    #127902
    Purpose
    WT Cas9 Vector targeting the 3' end of the mouse Ubf gene
    Depositor
    Mark Groudine
    Insert
    gRNA for Mouse 3' Ubf
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127902-plasmid-map-sequence-id-249774

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  15. pSB700-TTN-Puro

    Plasmid
    #128323
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against human TTN for activation
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128323-plasmid-map-sequence-id-255024

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  16. pZDonor-U6-myogenin stop codon gRNA

    Plasmid
    #69549
    Purpose
    Expresses guide RNA targeting near the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Insert
    Myogenin C terminus-targeting guide RNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69549-plasmid-map-sequence-id-122972

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  17. CMV-Lin28a-as

    Plasmid
    #120528
    Purpose
    ssRNA expression for dsRNA production
    Depositor
    Petr Svoboda
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    120528-plasmid-map-sequence-id-233984

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  18. Fly 2.0 pIDTBlue

    Plasmid
    #117418
    Purpose
    Drosophila melanogaster gene cloned into pIDTBlue vector for internal extraction control
    Depositor
    Dirk Dittmer
    Insert
    FLY 2.0
    Use
    Tags
    NA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    117418-plasmid-map-sequence-id-227235

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  19. CAG-EGFP-Lin28IR

    Plasmid
    #120517
    Purpose
    dsRNA expression
    Depositor
    Petr Svoboda
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120517-plasmid-map-sequence-id-234052

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  20. U6Lin28a-as

    Plasmid
    #120536
    Purpose
    ssRNA expression for dsRNA production
    Depositor
    Petr Svoboda
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120536-plasmid-map-sequence-id-233987

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  21. U6-Lin28a-s

    Plasmid
    #120535
    Purpose
    ssRNA expression for dsRNA production
    Depositor
    Petr Svoboda
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120535-plasmid-map-sequence-id-233980

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  22. CMV-Lin28a-s

    Plasmid
    #120527
    Purpose
    ssRNA expression for dsRNA production
    Depositor
    Petr Svoboda
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120527-plasmid-map-sequence-id-233968

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  23. CMV-Lin28aIR

    Plasmid
    #120524
    Purpose
    dsRNA expression
    Depositor
    Petr Svoboda
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120524-plasmid-map-sequence-id-233961

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  24. pCas9CR5

    Plasmid
    #120227
    Purpose
    pCas9CR4 with stop codon to prevent translation of ssrA degradation tag
    Depositor
    Christopher Reisch
    Insert
    SpCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Stop codon inserted before ssrA degradation tag
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120227-plasmid-map-sequence-id-233288

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  25. pJR288

    Plasmid
    #78550
    Purpose
    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.
    Depositor
    John Rossi
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6, EF1a
    Available since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    78550-plasmid-map-sequence-id-191096

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  26. pMP71-HLA-B15-His

    Plasmid
    #104848
    Purpose
    HLA-B150101 cloned as a His tag
    Depositor
    Sébastien Walchli
    Insert
    major histocompatibility complex, class I, B (HLA-B Human)
    Use
    Retroviral
    Tags
    his
    Expression
    Mammalian
    Mutation
    2 differences compared to original B15: L2->R …
    Promoter
    LTR
    Available since
    Dec. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104848-plasmid-map-sequence-id-201600

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  27. pJL16

    Plasmid
    #117425
    Purpose
    Expression of ARF3
    Depositor
    Richard Kahn
    Article
    et al unpublished
    Insert
    ARF3 (ARF3 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    117425-plasmid-map-sequence-id-228485

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  28. pmSod1-Q42L-N’His

    Plasmid
    #117708
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Q42L
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117708-plasmid-map-sequence-id-227535

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  29. pmSod1-Q123A-N’His

    Plasmid
    #117709
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Q123A
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117709-plasmid-map-sequence-id-227504

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  30. pmSod1-D83G-N’His

    Plasmid
    #117702
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    D83G
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117702-plasmid-map-sequence-id-227517

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  31. pHH0103 PQBP1 WW domain

    Plasmid
    #104287
    Purpose
    Bacterial expression of WW domain from PQBP1
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    PQBP1 WW domain (PQBP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104287-plasmid-map-sequence-id-203724

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  32. pHH0103 HLA-DQA2 WW domain

    Plasmid
    #104297
    Purpose
    Bacterial expression of WW domain from HLA-DQA2
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    HLA-DQA2 WW domain (HLA-DQA2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104297-plasmid-map-sequence-id-203688

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  33. TAP-Amotl2

    Plasmid
    #112834
    Purpose
    transient/stable overexpression
    Depositor
    Tomasz Prószyński
    Insert
    Amotl2 (Amotl2 Mouse)
    Use
    Tags
    TAP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    112834-plasmid-map-sequence-id-217933

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  34. pET28a-mxEH

    Plasmid
    #104977
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Deokkun Oh
    Insert
    MXAN_1644 (MXAN_1644 Myxococcus xanthus)
    Use
    Tags
    6 histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    May 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    104977-plasmid-map-sequence-id-202891

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  35. pHH0103 HYPC WW domain #2

    Plasmid
    #104232
    Purpose
    Bacterial expression of WW domain #2 from HYPC
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    HYPC WW domain #2 (PRPF40B Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    104232-plasmid-map-sequence-id-204196

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  36. GLT-1b delta 4

    Plasmid
    #98707
    Purpose
    mammalian expression of Glt1b delta 4 mutant
    Depositor
    Paul Rosenberg
    Insert
    Glt1b (Slc1a2 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    missing last 4 amino acids at the C-terminus. I52…
    Promoter
    Available since
    Sept. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    98707-plasmid-map-sequence-id-193958

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  37. 371-C

    Plasmid
    #97029
    Purpose
    His6-MBP-N10-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    His6 tag, MBP domain, N10 linker, and TEV cleavag…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97029-plasmid-map-sequence-id-182500

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  38. pCASCADE-udhA-zwf

    Plasmid
    #87153
    Purpose
    udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    udhA-zwf guide array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87153-plasmid-map-sequence-id-174933

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  39. pGEM-floxedEGFP-TRI2A

    Plasmid
    #74669
    Purpose
    floxedEGFP-TRI2A in between the homology regions left (HL) and right (HR) to Rosa26 murine locus
    Depositor
    Roberto Giovannoni
    Insert
    floxedEGFP-TRI2A in between the homology regions left (HL) and right (HR) to Rosa26 murine locus
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    pCAGGS
    Available since
    Feb. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    74669-plasmid-map-sequence-id-147558

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  40. pJK545

    Plasmid
    #74225
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    contains a silent mutation in Ala63 codon
    Promoter
    T7
    Available since
    July 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74225-plasmid-map-sequence-id-142021

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Showing: 3521 - 3560 of 8321 results