We narrowed to 2,365 results for: DAP

1. Anti-SAPAP2 [N459/52R]

   Plasmid

   #225398

   Purpose

   Mammalian Expression Plasmid of anti-SAPAP2 (Rat) IgG2a R-mAb. Derived from hybridoma N459/52.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-SAPAP2 (Rattus norvegicus) recombinant (Mouse) monoclonal antibody. (Dlgap2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Anti-SAPAP1 [N238/29]

   Plasmid

   #190303

   Purpose

   Mammalian Expression Plasmid of anti-SAPAP1 (Rat). Derived from hybridoma N238/29.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-SAPAP1 (Rattus norvegicus) recombinant Mouse monoclonal antibody (Dlgap1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Anti-SAPAP1 [N238/30]

   Plasmid

   #190304

   Purpose

   Mammalian Expression Plasmid of anti-SAPAP1 (Rat). Derived from hybridoma N238/30.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-SAPAP1 (Rattus norvegicus) recombinant Mouse monoclonal antibody (Dlgap1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

   Plasmid

   #197061

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Inserts

   Encapsulin
   Adaptor Protein
   

   Use

   CRISPR

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAVS1_TREtight-FRB-GEM23-Halo-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

   Plasmid

   #197065

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of FRB-GEM23-Halo and Adaptor Protein (FKBP-SNAP-vhhGFP4)

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Inserts

   I3-01
   Adaptor Protein
   

   Use

   CRISPR

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

   Plasmid

   #197064

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Inserts

   Encapsulin
   Adaptor Protein
   

   Use

   CRISPR

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYX233-b2-DHFR

   Plasmid

   #163759

   Purpose

   Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)-DHFR

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   b2-DHFR (Dhfr Mouse, Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

   Promoter

   GAL1

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-EMTB-TurboID-V5-puro

   Plasmid

   #190741

   Purpose

   A lentiviral plasmid encoding EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

   Depositor

   Jeremy Baskin

   Article

   Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

   Insert

   EMTB-TurboID-V5 (RPS14 EMTB is human ensconsin; TurboID is engineered BirA from E.coli, Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Microtubule binding-domain of ensconsin (amino ac…

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. FcD265A-IL2

   Plasmid

   #65419

   Purpose

   Mammalian expression plasmid for murine serum-persistent IL-2

   Depositor

   Dane Wittrup

   Article

   Zhu et al Cancer Cell. 2015 Apr 13;27(4):489-501.

   Insert

   FcD265A-IL2 (Il2 Mouse)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   D265A

   Promoter

   Modified CMV Promoter

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

10. lentiCRISPR v2-Nr4a1 double gRNAs

    Plasmid

    #162791

    Purpose

    Multiplex Guide RNAs to generate Nr4a1 knockout by CRISPR

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Multiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS416-Gal4-dCas9-VP64

    Plasmid

    #71128

    Purpose

    This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.

    Depositor

    Ronald Davis

    Article

    Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.

    Insert

    Gal4-dCas9-VP64
    

    Use

    Tags

    NLS n-terminal, N terminal Gal4 Activator domain,…

    Expression

    Yeast

    Mutation

    Promoter

    Tef1

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

    Plasmid

    #131143

    Purpose

    Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    mNeonGreen::3XFLAG::Crk (Crk Fly)
    

    Use

    Cre/Lox; Attb recombination site

    Tags

    3XFLAG and mNeonGreen

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVS1_TREtight-GEM4-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid

    #197062

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM4-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Inserts

    Encapsulin
    Adaptor Protein
    

    Use

    CRISPR

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAVS1_TREtight-GEM7-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid

    #197063

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM7-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Inserts

    Encapsulin
    Adaptor Protein
    

    Use

    CRISPR

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBridge-tyrosinase.σ1A

    Plasmid

    #197381

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-1 σ1A
    

    Use

    Tags

    GAL4-DNA binding domain fragment, HA tag, and SV4…

    Expression

    Yeast

    Mutation

    Contains an extra 42 nucleotides encoding 14 resi…

    Promoter

    ADH1 and MET25

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-HA-Nr4a1-AA

    Plasmid

    #160942

    Purpose

    Generation of retrovirus for the overexpression of Nr4a1 mutant (287C 288EAA)

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Nr4a1 (Nr4a1 Mouse)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Change Cys 287 Glu 288 into Ala Ala

    Promoter

    MSCV-LTR

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. shCCL20

    Plasmid

    #65096

    Purpose

    retroviral expression of CCL20 shRNA

    Depositor

    Pau Sancho-Bru

    Article

    Affo et al Gut. 2014 Nov;63(11):1782-92. doi: 10.11

    Insert

    Ccl20 shRNA (Ccl20 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

18. MSCV-NSD3-short del(280-342)

    Plasmid

    #72550

    Purpose

    expresses 3*FLAG tagged human NSD3-short with 280-342 aa deletion

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    280-342 aa deletion

    Promoter

    LTR

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-NSD3-short del(384-645)

    Plasmid

    #72549

    Purpose

    expresses 3*FLAG tagged human NSD3-short with 384-645 aa deletion

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    384-645 aa deletion

    Promoter

    LTR

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDF-casBCDE(+6)

    Plasmid

    #89728

    Purpose

    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.

    Depositor

    Konstantin Severinov, Konstantin Kuznedelov

    Article

    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Derivative CRISPR array containing a longer versi…

    Promoter

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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