We narrowed to 157,076 results for: Des

1. pcDNA 3.2/V5-DEST NET1A V5 WT

   Plasmid

   #69817

   Purpose

   Expresses NET1A-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   NET1A (NET1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-DEST42-ARF1-V5-His6

   Plasmid

   #67411

   Purpose

   Expression of hArf1 with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF1 (ARF1 Human)
   

   Tags

   His and V5

   Expression

   Bacterial

   Promoter

   T7/lacO

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA-Dest47- WTN23C11orf83-GFP

   Plasmid

   #65845

   Purpose

   Mammalian expression of the wild type N terminal part (N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

   Depositor

   Amos Bairoch

   Article

   Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

   Insert

   Transmembrane (23 AA) of UQCC3 (UQCC3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   WT Transmembrane

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST40-2XFL-Src-Y530EEI

   Plasmid

   #140323

   Purpose

   Expresses Flag-tagged human Src-Y530EEI in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   Y530EEI

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDest-1.7kbfabp6-eGFP-pA-CrymCherry

   Plasmid

   #159088

   Purpose

   LR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA inserts

   Depositor

   John Rawls

   Article

   Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

   Inserts

   1.7kb fabp6 promoter (fabp6 Zebrafish)
   eGFP
   polyA
   

   Use

   Fluorescent reporter

   Promoter

   1.7kb fabp6

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDest-312 (attR4-attR3)

   Plasmid

   #161897

   Purpose

   Multisite Gateway (attR4-attR3) vector for mammalian protein expression

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDest-302 (attR4-attR2)

   Plasmid

   #161894

   Purpose

   Multisite Gateway (attR4-attR2) vector for mammalian protein expression

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMMACE DEST CMV SpCas9

   Plasmid

   #206268

   Purpose

   DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   SpCas9
   

   Use

   CRISPR; Recombinant baculovirus production

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAD/CMV/V5-DEST-Rac1-2G

   Plasmid

   #66112

   Purpose

   Second generation Rac1 FRET biosensor for adenovirus production

   Depositor

   Olivier Pertz

   Article

   Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

   Insert

   Second generation Rac1 FRET biosensor
   

   Use

   Adenoviral

   Tags

   His-Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-hMeCP2-GFP-G273X

    Plasmid

    #48080

    Purpose

    Mammalian expression of GFP tagged MeCP2-G273X

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    expresses AT-Hook G273X

    Promoter

    CMV

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF-DEST51 GFP-PNRC1

    Plasmid

    #123295

    Purpose

    Mammalian expression of PNRC1 N-GFP

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    PNRC1 (PNRC1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEYFP-Pds1Δdestruction box(383)

    Plasmid

    #39848

    Depositor

    Jonathon Pines

    Article

    Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

    Insert

    Pds1 (PTTG1 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Δdestruction box = ΔR61-N68

    Available Since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST15.1-hRPN13 (285-407)

    Plasmid

    #61934

    Purpose

    Expression of hRPN13 (285-407) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hRPN13 (285-407) (ADRM1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Construct starts at residue 285

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

14. JP705: pMVP/SB/Neo-DEST

    Plasmid

    #121866

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Neo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. p1148-pCSDest-AcrIIA4-mCherry

    Plasmid

    #126204

    Purpose

    Mammalian expression of AcrIIA4 fused to mCherry

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Insert

    codon-optimized AcrIIA4
    

    Tags

    FLAG/NLS and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST-actc1b-ccdb-mKate2

    Plasmid

    #109731

    Purpose

    Multisite gateway destination vector for muscle specific expression of constructs tagged at the 3' end with mKate2

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Type

    Empty backbone

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-actc1b-mKate2-ccdb

    Plasmid

    #109732

    Purpose

    Multisite gateway destination vector for muscle specific expression of constructs tagged at the 5' end with mKate2

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Type

    Empty backbone

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST53-LRRK2-3XKD-Y1699C

    Plasmid

    #25051

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    "Kinase Dead" Lysine 1906 mutated to A…

    Available Since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

19. KK001: pMVP/SB/Hygro-DEST

    Plasmid

    #121867

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Hygro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST(frt)(ATG_V5-APEX2_noSTOP)

    Plasmid

    #168207

    Purpose

    Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection

    Depositor

    Markus Hafner

    Article

    Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

    Type

    Empty backbone

    Use

    Unspecified; Pdest gateway

    Tags

    V5 APEX2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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