We narrowed to 13,408 results for: RING;

1. pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP

   Plasmid

   #189630

   Purpose

   Expresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.

   Depositor

   Jerzy Szablowski

   Article

   Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Inserts

   Gaussia luciferase fused to Fc
   IRES-EGFP
   

   Use

   AAV, Cre/Lox, and Luciferase

   Tags

   Gluc and IgG1 Fc

   Expression

   Mammalian

   Mutation

   Promoter

   IRES and hSyn

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. mouse av full length

   Plasmid

   #217809

   Purpose

   Full length mouse integrin av expression

   Depositor

   Timothy Springer

   Article

   Yuxin et al bioRxiv 2024.01.26.577394

   Insert

   ITGAV (Itgav Mouse)
   

   Use

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcU6_3 MS2 sgRNA

   Plasmid

   #92394

   Purpose

   Chick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chick U6.3

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. GABA(A) receptor subunit a1SE

   Plasmid

   #49168

   Purpose

   pHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

   Depositor

   Tija Jacob, Stephen Moss

   Article

   Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do

   Insert

   GABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
   

   Use

   Tags

   myc and pHGFP (pHIuorin)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUGW-PK-hLC3∆G

   Plasmid

   #61461

   Purpose

   Express pHluorin-mKate2-hLC3∆G (PK-hLC3∆G), a negative control for pHluorin-mKate2-hLC3 (PK-hLC3), in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)

   Depositor

   Isei Tanida

   Article

   Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

   Insert

   PK-hLC3∆G (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUBC

   Available since

   Feb. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. mouse a2b full length

   Plasmid

   #217812

   Purpose

   Full length mouse integrin a2b expression

   Depositor

   Timothy Springer

   Article

   Yuxin et al bioRxiv 2024.01.26.577394

   Insert

   ITGA2B (Itga2b Mouse)
   

   Use

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. kanMX6-ins2

   Plasmid

   #195039

   Purpose

   pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATP1A1_plasmid_donor_RD

   Plasmid

   #86551

   Purpose

   Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 (ATP1A1 Human)
   

   Use

   CRISPR and TALEN; Co-selection via hdr using ouab…

   Tags

   Expression

   Mammalian

   Mutation

   Q118R, N129D

   Promoter

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 His-GFP-EWSR1-myc-His

   Plasmid

   #46385

   Purpose

   expresses EGFP tagged EWSR1 in mammalian cells

   Depositor

   Heinz Gehring

   Article

   Pahlich et al Proteins. 2008 Sep;72(4):1125-37. doi: 1

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   EGFP, His, and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

10. mouse b5 full length

    Plasmid

    #217815

    Purpose

    Full length mouse integrin b5 expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGB5 (Itgb5 Mouse)
    

    Use

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. natMX6-ins5

    Plasmid

    #195042

    Purpose

    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    NatR
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRP1MX6-ins4

    Plasmid

    #195041

    Purpose

    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    TRP1
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. human a5 full length

    Plasmid

    #217818

    Purpose

    Full length human integrin a5 expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGA5 (ITGA5 Human)
    

    Use

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. his3MX6-ins3

    Plasmid

    #195040

    Purpose

    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    His5+
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNOC_dCas9_sgRNA NC

    Plasmid

    #176259

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    H840A and D10A mutations on spCas9 to inactivate …

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-hs-HRAS

    Plasmid

    #52730

    Purpose

    retroviral expression of human HRAS

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    HRAS (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pNOC_dfnCas12a_crRNA NC

    Plasmid

    #176256

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can be

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    D917A and E1006A mutations to inactivate the endo…

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNOC_hfnCas12a-Nlux_crRNA NR1

    Plasmid

    #176244

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Ribosomal subunit

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. EW482 CMV-TO-ADAR2dd(E488Q, T501A)-PUF-9R-MARIAcomp(V241D, H243M) [D241C, M243Q] (FLP-IN)

    Plasmid

    #236128

    Purpose

    Plasmid encoding ADAR2dd attached to PUF-9R with deimmunizing and function-restoring mutations under control of CMV promoter with two TetR binding sites

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    ADAR2dd-PUF-9R (ADARB1 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q, T501A in ADAR2dd; V241C, H243Q in PUF-9R

    Promoter

    CMV promoter with two TetR binding sites

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. PD2529 human beta1 ecto

    Plasmid

    #221398

    Purpose

    Mammalian expression of human integrin beta1 ectodomain

    Depositor

    Timothy Springer

    Article

    Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

    Insert

    integrin beta1 ectodomain (ITGB1 Human)
    

    Use

    Tags

    AVI tag, HRV3C cleavage site, basic coil, HA tag,…

    Expression

    Mammalian

    Mutation

    codon optimized for human mature _1 residues Q1 t…

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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