We narrowed to 14,729 results for: nans

1. pET21-pelB-LaM-6

   Plasmid

   #172771

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-6
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. MDO2-c005

   Plasmid

   #173106

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   MACROD2 (MACROD2 Human)
   

   Use

   Tags

   Nanoluc

   Expression

   Bacterial

   Mutation

   K7-D243

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GOMO-GFP-LUC

   Plasmid

   #60976

   Purpose

   Rapid selection of recombinant marker-free bioluminescent rodent malaria parasites

   Depositor

   Olivier Silvie

   Article

   Manzoni et al Sci Rep. 2014 Apr 23;4:4760. doi: 10.103

   Inserts

   GFP-Luciferase fusion
   mCherry
   hDHFR-yFCU
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. K8.1Pr-pGL4.16

   Plasmid

   #131037

   Purpose

   Firefly Luciferase reporter for K8.1 promoter from KSHV

   Depositor

   Britt Glaunsinger

   Article

   Nandakumar et al PLoS Pathog. 2019 May 16;15(5):e1007774.

   Insert

   KSHV K8.1 100 bp promoter
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. SI16-Kit a

   Plasmid

   #58948

   Purpose

   expresses recombinant mouse anti-Kit a receptor (zebrafish) IgG1 antibody in mammalian cells

   Depositor

   Gavin Wright

   Article

   Crosnier et al BMC Biol. 2010 . 8():76.

   Insert

   Kit receptor a mouse monoclonal antibody (kita Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

6. PKC-delta-IRES-hrGFP

   Plasmid

   #73414

   Purpose

   Mammalian expression of mouse PKC-delta and hrGFP

   Depositor

   Raymond Swanson

   Article

   Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

   Insert

   PKC-delta (Prkcd Mouse)
   

   Use

   Tags

   3xFLAG and IRES-hrGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pET21-pelB-LaG-27

   Plasmid

   #172752

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-27
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-EF1a-MTS-FLAG-LOV*

   Plasmid

   #202208

   Purpose

   Plasmid for the generation of a lentiviral vector encoding the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells

   Depositor

   Thomas Muir

   Article

   Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

   Insert

   Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24
   

   Use

   Lentiviral

   Tags

   FLAG and LOV*

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUASTLOTattB_mCherry::Nrv1::vhhGFP4

   Plasmid

   #163926

   Purpose

   Expression of GrabFP-B-intracellular under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-2Aneo v2

    Plasmid

    #80033

    Purpose

    Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

    Depositor

    Hiroyuki Konishi

    Article

    Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; A platform vector to construct t…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-2Aneo

    Plasmid

    #80032

    Purpose

    Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

    Depositor

    Hiroyuki Konishi

    Article

    Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; A platform vector to construct t…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Po_CyRPA-Cd4-bio-His

    Plasmid

    #126829

    Purpose

    Expression of recombinant P. ovale protein in mammalian cells

    Depositor

    Gavin Wright

    Article

    Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

    Insert

    CyRPA
    

    Use

    Tags

    ratCd4(d3+4)-bio-6His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Pm_CyRPA-Cd4-bio-His

    Plasmid

    #126817

    Purpose

    Expression of recombinant P. malariae protein in mammalian cells

    Depositor

    Gavin Wright

    Article

    Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

    Insert

    CyRPA (PmUG01_05040800 Plasmodium malariae)
    

    Use

    Tags

    ratCd4(d3+4)-bio-6His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21-pelB-LaG-24

    Plasmid

    #172750

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-24
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21-pelB-LaG-9

    Plasmid

    #172742

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-9
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNOC-CRISPR-35S

    Plasmid

    #99861

    Purpose

    Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Cas9-Nlux

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21-pelB-LaG-8

    Plasmid

    #172761

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-8
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21-pelB-LaG-5

    Plasmid

    #172760

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-5
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a Gx-NL2

    Plasmid

    #169107

    Purpose

    Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUASTLOTattB_vhhGFP4::Vkg::mCherry

    Plasmid

    #163929

    Purpose

    Expression of GrabFP-ECM under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 nanobody with Viking (Collagen IV) protein and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Mutation

    G1767D

    Promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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