159,333 results

1. pSB-BbsI-sgRNA

   Plasmid

   #203359

   Purpose

   Contains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backbone

   Depositor

   Jonathan Brunger

   Article

   Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

   Insert

   BbsI Cut Sites
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDB5321

   Plasmid

   #204831

   Purpose

   An integrating plasmid containing both the inducible enotetSW3 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

   Depositor

   Li-Lin Du

   Article

   Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

   Inserts

   tetR
   mECitrine
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CMV promoter and enotetSW3 promoter

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-hs-EGR1

   Plasmid

   #52724

   Purpose

   retroviral expression of human EGR1

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   EGR1 (EGR1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #191797

   Purpose

   vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized dCas13d
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   R239A, H244A, R858A, H863A

   Promoter

   CBh, SV40, hU6

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Zebrabow-GateDest

   Plasmid

   #118221

   Purpose

   For cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.

   Depositor

   Alex Schier, Albert Pan

   Article

   Pan et al Development. 2013 Jul;140(13):2835-46. d

   Insert

   Brainbow 1.0 L - ccdB survival cassette
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. hKCNQ5(WT).ires.GFP-pcDNA3

   Plasmid

   #204360

   Purpose

   Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

   Depositor

   Aguan Wei

   Article

   Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

   Insert

   KCNQ5 (KCNQ5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/T7 RNApol

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pInducer-myc-ERK2-R67S/S153D

   Plasmid

   #213597

   Purpose

   Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D cDNA in mammilian cells

   Depositor

   Channing Der

   Article

   Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   R67S, S153D

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

   Plasmid

   #199371

   Purpose

   Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   anti-ALFA nanobody
   

   Use

   Tags

   14xHis-Avi

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T5-LacO

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLIK-rABE-Flag

   Plasmid

   #191383

   Purpose

   Doxycycline inducible expression of RNA A-to-I editor rABE

   Depositor

   Stephen Floor

   Article

   Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

   Insert

   rABE-Flag
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   tet-inducible promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG/ATP9A-HA

    Plasmid

    #209213

    Purpose

    Mammalian expression of ATP9A

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP9A (ATP9A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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