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pGL3-U6-sgRNA_SRD5A1-mcherry

Plasmid

#159780

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting human SRD5A1 gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting SRD5A1 gene (SRD5A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop

Plasmid

#169369

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

CPIJ039653

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pVMG0146_Cq-U6-1_2xBbsI-gRNA

Plasmid

#169238

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

Cq-U6-1_2xBbsI-gRNA

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

CPIJ039653

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pVMG0149_Cq-U6-6_2xBbsI-gRNA

Plasmid

#169323

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0149_Cq-U6-6_2xBbsI-gRNA

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

CPIJ039596

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSilencer2.1-U6-3JB1R+6

Plasmid

#190842

Purpose

For mammalian expression of 3JB1R+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

3JB1R_AP3+6bp (A Dual-Specificity aptamer targeting OGT and GFP)

Use

Affinity Reagent/ Antibody and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSilencer2.1-U6-17-6

Plasmid

#190827

Purpose

For mammalian expression of 17-6, a non-aptamer RNA. Expression is driven by a U6 promoter.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

17-6 (Non-aptamer RNA)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSTBlue-U6-sgRNA(tdTomato)

Plasmid

#47871

Purpose

This plasmid contains expression cassette for sgRNA targeting tdTomato.

Depositor

James Thomson, Erik Sontheimer

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Insert

sgRNA-tdTomato

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pBS U6 WIP RNAi

Plasmid

#11774

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WIP RNAi (WIPF1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pGL3-U6-pegRNA_FANCF-EGFP

Plasmid

#159785

Purpose

S. pyogenes pegRNA for C to A mutation at the FANCF site of human cells using prime editing

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of pegRNA targeting FANCF gene (FANCF Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA_VEGFA-mcherry

Plasmid

#159790

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting human VEGFA gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting VEGFA gene (VEGFA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-U6-shR-Scr

Plasmid

#19800

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 . 8():77.

Insert

scrambled shRNA

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
BC1364-mouse U6-sgH2B

Plasmid

#164043

Purpose

U6-driven sgRNA targeting H2B

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

H2B targeting sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

mouse U6

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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U6>Control(F+E)

Plasmid

#60006

Purpose

U6 promoter driving control (F+E) sgRNA

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

Control (F+E) sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ciinte.U6

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop

Plasmid

#169370

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

CPIJ039596

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-U6-sgRNA_EMX1-mcherry

Plasmid

#159784

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting human EMX1 gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting EMX1gene (EMX1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-pegRNA_HOXD13_CtoT-EGFP

Plasmid

#159792

Purpose

S. pyogenes pegRNA for C to T mutation at the HOXD13 site of mouse cells using prime editing

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of pegRNA_HOXD13_CtoT targeting HOXD13 gene (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-U6-pegRNA_HOXD13_CtoG-EGFP

Plasmid

#159791

Purpose

S. pyogenes pegRNA for C to G mutation at the HOXD13 site of mouse cells using prime editing

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of pegRNA_HOXD13_CtoG targeting HOXD13 gene (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-U6-sgRNA_HOXD13-mcherry

Plasmid

#159793

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting mouse HOXD13 gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting HOXD13 gene (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRNA-U6-TuDmiR122 seedsupplemental

Plasmid

#35668

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

TuD miR-122seedsupplemental

Use

Tags

Expression

Mutation

Promoter

U6

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-mmDNMT1

Plasmid

#205635

Purpose

reRNA guide targeting Mus Musculus DNMT1

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-mmDNMT1 (Dnmt1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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