We narrowed to 359 results for: pRSET

1. pRSET FLII275Pglu-4.6m

   Plasmid

   #13565

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIPglu F16A G275S-ECFP-K276R
   

   Use

   Tags

   Citrine

   Expression

   Bacterial

   Mutation

   F16A, ECFP insert in mglB

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSET FLIPE-10u

   Plasmid

   #13538

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207R
   

   Use

   Tags

   ECFP and Venus

   Expression

   Bacterial

   Mutation

   A207R in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSET FLIPglu-2uDelta13

   Plasmid

   #13561

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIPglu F16F D152A Delta13
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   F16A, D152A in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSET a sfGCaMP6s-T78H

   Plasmid

   #100023

   Purpose

   Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   sfGCaMP6s-T78H
   

   Use

   Tags

   6x HIS tag and Xpress_EK tag

   Expression

   Bacterial

   Mutation

   cpEGFP replaced with superfolder cpGFP and T78H m…

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET.HaloTag.v7.CoOp.Cys-less

   Plasmid

   #195487

   Purpose

   Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Insert

   HaloTag.v7
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C61T, C262V

   Promoter

   T7

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSETa-mEos4b-V69T

   Plasmid

   #98572

   Purpose

   photoconvertible fluorescent protein mEos4b-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression

   Depositor

   Ulrike Endesfelder

   Article

   Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

   Insert

   mEos4b-V69T
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSET-A2-77p-Venus

   Plasmid

   #64856

   Purpose

   Expresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tag

   Depositor

   Sriram Neelamegham

   Article

   Gogia et al PLoS One. 2015 May 18;10(5):e0126556. do

   Insert

   VWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Synthetic, Human)
   

   Use

   Tags

   7X His and Venus

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSETa-mKikGR-V69T

   Plasmid

   #98574

   Purpose

   photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

   Depositor

   Ulrike Endesfelder

   Article

   Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

   Insert

   mKikGR-V69T
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSETB-roGFP1-R10

   Plasmid

   #83310

   Purpose

   Inducible expression of redox sensitive GFP in bacteria

   Depositor

   S. James Remington

   Article

   Cannon et al Protein Sci. 2006 Jan;15(1):45-57. Epub

   Insert

   roGFP1-R10
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C48S/Q80R/S147C/Q204C/F223R/S202K/K41D

   Promoter

   T7

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pRSET FLIPmal-25u

    Plasmid

    #14480

    Depositor

    Wolf Frommer

    Article

    Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 9

    Insert

    FLIPmal
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    W230A

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

11. HOXD12 (murine) pRSETa

    Plasmid

    #8554

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXD12 (Hoxd12 Mouse)
    

    Use

    Tags

    HIS and XPress

    Expression

    Bacterial

    Mutation

    Fusion sequence is: ggatccATG

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pRSET-GST-05230

    Plasmid

    #67874

    Purpose

    E.coli expression vector derived from pRSET encoding shy1 (um05230) with a N-terminal GST-fusion.

    Depositor

    Armin Djamei

    Article

    Rabe et al Mol Microbiol. 2013 Jul;89(1):179-88. do

    Insert

    shy1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET FLIPE-100u

    Plasmid

    #13539

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E S118W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    S118W in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSET-spa-GCaMP6f

    Plasmid

    #67563

    Purpose

    bacterial expression of a photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6f
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pRSET FLII32Pglu-2.2m

    Plasmid

    #13566

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A P32S-ECFP-D33R
    

    Use

    Tags

    6xHis and Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSET FLIPmal-225u

    Plasmid

    #14481

    Depositor

    Wolf Frommer

    Article

    Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 9

    Insert

    FLIPmal
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    W62A

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pRSET FLIPE intramol-1m

    Plasmid

    #13551

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIP-E intramol A207W (gltI E. coli)
    

    Use

    Tags

    EYFP

    Expression

    Bacterial

    Mutation

    A207W, ECFP insert in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSET FLII12Pglu-600uDelta4

    Plasmid

    #13567

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    FLIPglu F16A Y12S-ECFP-D13R, 4aa linker
    

    Use

    Tags

    Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in mglB

    Promoter

    Available since

    Feb. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSETb-LSS-mEGFP

    Plasmid

    #112942

    Purpose

    Bacterial expression vector for LSS-EGFP

    Depositor

    Dorus Gadella

    Insert

    LSSS-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSETb-LSS-SGFP2

    Plasmid

    #112939

    Purpose

    Bacterial expression vector for LSS-SGFP2

    Depositor

    Dorus Gadella

    Insert

    LSS-SGFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge