3,636 results

1. pRSFDuet-1 IntS8 AA 1-308

   Plasmid

   #196905

   Purpose

   Expresses His-tagged Drosophila IntS8 amino acids 1-308

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   Integrator subunit 8 (IntS8 Fly)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT-EGFP-Actin5c

   Plasmid

   #15312

   Depositor

   Ron Vale

   Article

   Rogers et al J Cell Biol. 2003 Sep 15. 162(6):1079-88

   Insert

   Actin5c (Act5C Fly)
   

   Tags

   EGFP and His

   Expression

   Insect

   Available Since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pUASp YFP Rabx6 WT

   Plasmid

   #53479

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rabx6 (RabX6 Fly)
   

   Tags

   YFP

   Expression

   Insect

   Promoter

   GAL4

   Available Since

   May 12, 2014

   Availability

   Academic Institutions and Nonprofits only

4. 10XUAS IVS ChrimsonR mCherry_tr

   Plasmid

   #111547

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR_mCherry_trafficked
   

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. SCP2-CMVenh-luc

   Plasmid

   #22230

   Depositor

   James Kadonaga

   Article

   Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

   Insert

   SCP2 (super core promoter 2)-CMVenh luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB1H2wL-Prd

   Plasmid

   #18040

   Depositor

   Scot Wolfe

   Article

   Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

   Insert

   Paired (prd Fly)
   

   Expression

   Bacterial

   Mutation

   Paired is simply used as an insert between Kpn1 a…

   Available Since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. DmTmem63

   Plasmid

   #136598

   Purpose

   Expresses the mechanosensitive channel Drosophila melanogaster Tmem63 in mammalian cells

   Depositor

   Ardem Patapoutian

   Article

   Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

   Insert

   DmTMEM63
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFD7

   Plasmid

   #73916

   Purpose

   gRNA vector for AsCpf1

   Depositor

   Simon Bullock

   Article

   Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

   Insert

   tRNA:hAsCpf1-gRNA:tRNA
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   dU6:3

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAFW-Ago2

   Plasmid

   #50554

   Purpose

   Expression of FLAG tagged Ago2 in S2 cell

   Depositor

   Yukihide Tomari

   Article

   Iwasaki et al Mol Cell. 2010 Jul 30;39(2):292-9. doi:

   Insert

   Argonaute2 (AGO2 Fly)
   

   Tags

   FLAG

   Expression

   Insect

   Promoter

   Actin5C

   Available Since

   Feb. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCFJ1209

    Plasmid

    #44491

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, NeoR]
    

    Use

    Gateway expression clone

    Available Since

    April 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

    Plasmid

    #217513

    Purpose

    Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
    

    Expression

    Insect

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUWR-Cad-Venus

    Plasmid

    #58328

    Purpose

    Destination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    Cad-Venus
    

    Expression

    Insect

    Promoter

    poly-ubiquitin

    Available Since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

13. PGRP-SA

    Plasmid

    #21055

    Depositor

    Johann Deisenhofer

    Article

    Chang et al PLoS Biol. 2004 Sep . 2(9):E277.

    Insert

    PGRP-SA (PGRP-SA Fly)
    

    Tags

    His

    Expression

    Insect

    Available Since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

    Plasmid

    #199197

    Purpose

    Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int-BlastR
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUAST_NSlmb-vhhGFP4

    Plasmid

    #35575

    Depositor

    Markus Affolter

    Article

    Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

    Insert

    NSlmb-vhhGFP4 (slmb Fly)
    

    Expression

    Insect

    Available Since

    April 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. KRigor980

    Plasmid

    #129763

    Purpose

    Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    R210A

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-HemmarG

    Plasmid

    #31221

    Depositor

    Yuh-Nung Jan

    Article

    Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

    Insert

    CD4-tdGFP (CD4 Fly, Human, Aequorea victoria)
    

    Tags

    CD4 and EGFP/GFP

    Expression

    Insect

    Mutation

    Fusion of signal peptide of Drosophila Akh gene, …

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMT-Diaphanous-GFP

    Plasmid

    #24287

    Depositor

    Ron Vale

    Article

    Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

    Insert

    Diaphanous (dia Fly)
    

    Tags

    GFP

    Expression

    Insect

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pMT-HA-STX1

    Plasmid

    #89310

    Purpose

    Expresses HA tagged syntaxin 1 in Drosophila cells

    Depositor

    Andrew Peden

    Insert

    STX1 (Syx1A Fly)
    

    Tags

    HA tag

    Expression

    Insect

    Promoter

    P-MT (Metallothionein)

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRB17

    Plasmid

    #52527

    Purpose

    serves as template for PCR-mediated fusion of U6-promoter with sgRNA

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Drosophila U6C promoter
    

    Use

    CRISPR; Blunt cloning kit

    Tags

    T7-promoter

    Expression

    Insect

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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