3,636 results

1. pMT-EGFP-Actin5c

   Plasmid

   #15312

   Depositor

   Ron Vale

   Article

   Rogers et al J Cell Biol. 2003 Sep 15. 162(6):1079-88

   Insert

   Actin5c (Act5C Fly)
   

   Use

   Tags

   EGFP and His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pMT-HA-STX1

   Plasmid

   #89310

   Purpose

   Expresses HA tagged syntaxin 1 in Drosophila cells

   Depositor

   Andrew Peden

   Insert

   STX1 (Syx1A Fly)
   

   Use

   Tags

   HA tag

   Expression

   Insect

   Mutation

   Promoter

   P-MT (Metallothionein)

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pB1H2wL-Prd

   Plasmid

   #18040

   Depositor

   Scot Wolfe

   Article

   Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

   Insert

   Paired (prd Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Paired is simply used as an insert between Kpn1 a…

   Promoter

   Available since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RP156 pUAST EGFP:Gr21a

   Plasmid

   #59524

   Purpose

   N-terminal fusion of EGFP to Drosophila melanogaster Gr21a

   Depositor

   Leslie Vosshall

   Article

   Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

   Insert

   EGFP:Gr21 (Gr21a Fly)
   

   Use

   Tags

   EGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

5. KRigor980

   Plasmid

   #129763

   Purpose

   Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   R210A

   Promoter

   Available since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAFW-Ago2

   Plasmid

   #50554

   Purpose

   Expression of FLAG tagged Ago2 in S2 cell

   Depositor

   Yukihide Tomari

   Article

   Iwasaki et al Mol Cell. 2010 Jul 30;39(2):292-9. doi:

   Insert

   Argonaute2 (AGO2 Fly)
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   Feb. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUAST_NSlmb-vhhGFP4

   Plasmid

   #35575

   Depositor

   Markus Affolter

   Article

   Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

   Insert

   NSlmb-vhhGFP4 (slmb Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

   Plasmid

   #172066

   Purpose

   Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   mTagBFP2(11x7)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pDmVasa

   Plasmid

   #165783

   Purpose

   Drosohphila vasa germline promoter and 5'UTR

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Vasa promoter
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBac{3XP3::EGFP, Pactin::Ptrsps}

    Plasmid

    #86861

    Purpose

    Expresses EGFP in eyes and ubiquitous expression of P-transposase

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Insert

    pACNNTNPII-S129A
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. OA-1043

    Plasmid

    #164586

    Purpose

    clone in U6 and DR

    Depositor

    Omar Akbari

    Article

    Sun et al J Vis Exp. 2021 Feb 5;(168). doi: 10.379

    Insert

    U6 and DR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDropIn-T2A-split-intein-Gal4[1-20]-N-int

    Plasmid

    #199195

    Purpose

    Insert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDropIn-T2A-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199196

    Purpose

    Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMT-mCherry-Patronin

    Plasmid

    #31753

    Depositor

    Ron Vale

    Article

    Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

    Insert

    Patronin (Patronin Fly)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pUAST-attB-3xHA-V5-RpL10Ab

    Plasmid

    #125223

    Purpose

    3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

    Depositor

    Herman Dierick

    Article

    Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

    Insert

    RpL10Ab (RpL10Ab Fly)
    

    Use

    Tags

    3x HA, V5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST-HemmarG

    Plasmid

    #31221

    Depositor

    Yuh-Nung Jan

    Article

    Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

    Insert

    CD4-tdGFP (CD4 Human, Aequorea victoria, Fly)
    

    Use

    Tags

    CD4 and EGFP/GFP

    Expression

    Insect

    Mutation

    Fusion of signal peptide of Drosophila Akh gene, …

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen

    Plasmid

    #196974

    Purpose

    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tag

    Depositor

    Lukas Kapitein

    Article

    Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Use

    Tags

    GST and mNeonGreen

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAC-dCLK

    Plasmid

    #33153

    Depositor

    Michael Rosbash

    Article

    McDonald et al Mol Cell Biol. 2001 Feb;21(4):1207-17.

    Insert

    CLK (Clk Fly)
    

    Use

    Retroviral

    Tags

    6xHIS and V5

    Expression

    Insect

    Mutation

    Promoter

    Actin 5C

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pRB17

    Plasmid

    #52527

    Purpose

    serves as template for PCR-mediated fusion of U6-promoter with sgRNA

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Drosophila U6C promoter
    

    Use

    CRISPR; Blunt cloning kit

    Tags

    T7-promoter

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Ftz-M3

    Plasmid

    #11245

    Depositor

    Robin Reed

    Article

    Zhou et al Proc Natl Acad Sci U S A. 2002 Sep 17. 9

    Insert

    Ftz-M3
    

    Use

    Tags

    3 MS2 hairpins

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2006

    Availability

    Academic Institutions and Nonprofits only