Addgene

We narrowed to 470 results for: Chuk

Showing: 321 - 360 of 470 results

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pAAV.hSynapsin.SF-iGluSnFR.A184V

Plasmid

#106175

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Tags

Expression

Mutation

GltI: A184V

Promoter

hSynapsin

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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MAC-CTSS

Plasmid

#172415

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Cathepsin S (CTSS Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET-His6-SUMO-MproC145A

Plasmid

#172653

Purpose

Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO

Depositor

Stephen Harrison

Article

MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25

Insert

SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Tags

6xHis Tag and SUMO

Expression

Bacterial

Mutation

Catalytic Cysteine 145 mutated to Alanine

Promoter

T7

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap-FLEX.SF-iGluSnFR.S72A

Plasmid

#106182

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Tags

Expression

Mutation

GltI: S72A

Promoter

hSynapsin

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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MAC-CLEC4E

Plasmid

#172412

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

C-type lectin domain family 4 member E (CLEC4E Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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OR2M4_Deletion_gRNA

Plasmid

#195194

Purpose

dual gRNAs for deletion of OR2M4 in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

OR2M4 dual gRNA (OR2M4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG-FLEX.SF-iGluSnFR.A184V

Plasmid

#106187

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Tags

Expression

Mutation

GltI: A184V

Promoter

CAG

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.GFAP.SF-iGluSnFR.A184V

Plasmid

#106193

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Tags

Expression

Mutation

GltI: A184V

Promoter

GFAP

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-iGluSnFR.S72A

Plasmid

#106194

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Tags

Expression

Mutation

GltI: S72A

Promoter

GFAP

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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mito-yHS1-M7A,H102A

Plasmid

#159165

Purpose

Mitochondrial labile heme reporter

Depositor

Amit Reddi

Article

Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

Insert

mito-yHS1-M7A,H102A

Use

Tags

COX4 pre-sequence

Expression

Yeast

Mutation

Mutated both Met 7 and His 102 of the cyt b562 mo…

Promoter

GPD

Available since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV.CAG-FLEX.SF-iGluSnFR.S72A

Plasmid

#106188

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Tags

Expression

Mutation

GltI: S72A

Promoter

CAG

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pNK7185

Plasmid

#219765

Purpose

Vector for expression PzPKS2 in yeast (P.pastoris)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PzPKS2

Use

Tags

Expression

Yeast

Mutation

Promoter

pGAP

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK7186

Plasmid

#219758

Purpose

Vector for expression PpASCL in mammalian cells (HEK293NT)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PpASCL

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK7179

Plasmid

#219757

Purpose

Vector for expression HmS in mammalian cells (HEK293NT)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

HmS

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV4-HA-p31(comet)-S102A

Plasmid

#221698

Purpose

Mammalian expression of HA-tagged human p31(comet) (S102A)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2BP1

Use

Tags

Expression

Mammalian

Mutation

S102A

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-RNF8-R42A

Plasmid

#221702

Purpose

Lentiviral expression of human RNF8 (R42A) in mammalian cells

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Lentiviral

Tags

Expression

Mutation

R42A

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-RNF8-C403S

Plasmid

#221703

Purpose

Lentiviral expression of human RNF8 (C403S) in mammalian cells

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Lentiviral

Tags

Expression

Mutation

C403S

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-RNF8-S157A

Plasmid

#221704

Purpose

Lentiviral expression of human RNF8 (S157A) in mammalian cells

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Lentiviral

Tags

Expression

Mutation

S157A

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG-MAD2-RQ

Plasmid

#221691

Purpose

Mammalian expression of FLAG-tagged human MAD2 (R133E/Q134A)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2

Use

Tags

Expression

Mammalian

Mutation

R133E, Q134A

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG-MAD2

Plasmid

#221690

Purpose

Mammalian expression of FLAG-tagged human MAD2

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG-p31(comet)

Plasmid

#221692

Purpose

Mammalian expression of FLAG-tagged human p31(comet)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2BP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG-p31(comet)-QF

Plasmid

#221693

Purpose

Mammalian expression of FLAG-tagged human p31(comet) (Q83A/F191A)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2BP1

Use

Tags

Expression

Mammalian

Mutation

Q83A, F191A

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG-RNF8

Plasmid

#221695

Purpose

Mammalian expression of FLAG-tagged human RNF8

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK7184

Plasmid

#219756

Purpose

Vector for expression PzPKS2 in mammalian cells (HEK293NT)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PzPKS2

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK7189

Plasmid

#219759

Purpose

Vector for expression At4CL2 in mammalian cells (HEK293NT)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

At4CL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

pEF1

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK7191

Plasmid

#219764

Purpose

MoClo-compatible Level M for expression PpASCL in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PpASCL

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK7181

Plasmid

#219766

Purpose

Vector for expression HmS in yeast (P.pastoris)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

HmS

Use

Tags

Expression

Yeast

Mutation

Promoter

pGAP

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK7182

Plasmid

#219767

Purpose

Vector for expression PpASCL in yeast (P.pastoris)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PpASCL

Use

Tags

Expression

Yeast

Mutation

Promoter

pGAP

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK7188

Plasmid

#219768

Purpose

Vector for expression Pv4CL1 in yeast (P.pastoris)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

Pv4CL1

Use

Tags

Expression

Yeast

Mutation

Promoter

pGAP

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-C Sars-CoV-2 E P71L

Plasmid

#194804

Purpose

Sars-CoV-2 E_P71L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

E_P71L (E Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

P71L

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MAC-C Sars-CoV-2 N_D3L

Plasmid

#194806

Purpose

Sars-CoV-2 N_D3L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_N_D3L (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

N_D3L

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MAC-C Sars-CoV-2 N_D63G

Plasmid

#194807

Purpose

Sars-CoV-2 N_D63G in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_D63G (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

D63G

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MAC-C Sars-CoV-2 N_R203M

Plasmid

#194810

Purpose

Sars-CoV-2 N_R203M in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_R203M (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

R203M

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MAC-C Sars-CoV-2 N_S235F

Plasmid

#194811

Purpose

Sars-CoV-2 N_S235F in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_S235F (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

S235F

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
HA-C Sars-CoV-2 N_T205I

Plasmid

#194812

Purpose

Sars-CoV-2 N_T205I in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_T205I (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

T205I

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MAC-C Sars-CoV-2 NSP12_P314L

Plasmid

#194813

Purpose

Sars-CoV-2 NSP12_P314L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP12_P314L (ORF1ab Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

P314L

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Chr1q_Cassette-Proximal_gRNA

Plasmid

#195127

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting location immediately downstream of Chr1q_cassette-integration_gRNA, to be used for cassette deletion controls

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDON223 Spike L241-L242-A243-

Plasmid

#180070

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike L241-L242-A243- (S SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike L241-L242-A243-

Promoter

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA4/TO-BcRF1-ORF24-2xStrep

Plasmid

#138454

Purpose

Expresses BcRF1-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1-ORF24 (BcRF1, ORF24 EBV/KSHV)

Use

Tags

Strep

Expression

Mammalian

Mutation

BcRF1 amino acids 1-178 fused to ORF24 amino acid…

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-UL87-ORF24-2xStrep

Plasmid

#138455

Purpose

Expresses UL87-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

UL87-ORF24 (UL87, ORF24 HCMV/KSHV)

Use

Tags

Strep

Expression

Mammalian

Mutation

UL87 amino acids 1-248 fused to ORF24 amino acids…

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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