Skip to main content
Addgene
Showing: 401 - 450 of 2096 results

  7. pLL3.7-EF-EYFP-YAP1_WW-PolyA

    Plasmid
    #112290
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) WW mutant. WQDP (199-202) in the first WW domain was changed to AQDA, and the WLDP (258-261) of the second WW domain was changed to ALDA
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Human YAP1-2gamma with mutations in the WW domain…
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112290-plasmid-map-sequence-id-213664

    Enlarge

  12. pLL3.7-EF-EYFP-YAP1_delta5C-PolyA

    Plasmid
    #112289
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the protein
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Human YAP1-2gamma lacking the last 5 Amino acids …
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112289-plasmid-map-sequence-id-213521

    Enlarge

  13. pLL3.7-EF-EYFP-YAP1_Y3F-PolyA

    Plasmid
    #112288
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) Y3F mutant _ tyrosines 341, 357 and 394 corresponding to tyrosines 391, 407 and 444 in this isoform
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    3 Tyrosines (341, 357 and 394 corresponding to ty…
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112288-plasmid-map-sequence-id-213531

    Enlarge

  26. R777-E360 Hs.YAP1-nostop

    Plasmid
    #70644
    Purpose
    Gateway ORF clone of human YAP1 [NM_001130145.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    YAP1 (YAP1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70644-plasmid-map-sequence-id-126093

    Enlarge

  35. pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227901
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    MAP1LC3B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227901-plasmid-map-sequence-id-457382

    Enlarge

  36. LEGO-pDHS-AP1x6-GM55

    Plasmid
    #217416
    Purpose
    Luciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter, and a chromatin priming element. Alias: LEGO-GM55-AP-1x6-pDHS(IL-3)
    Depositor
    Peter Cockerill
    Insert
    Luciferase, GFP
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    6 AP-1 sites, Human -55 to +28 minimal CSF2 promo…
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217416-plasmid-map-sequence-id-434961

    Enlarge

  37. LEGO-pDHS-AP1x6-DUSP5

    Plasmid
    #217418
    Purpose
    Luciferase expression vector with 6 AP-1 sites and the full length DUSP5 promoter, and a chromatin priming element. Alias: LEGO-DUSP5p-AP-1x6
    Depositor
    Peter Cockerill
    Insert
    Luciferase, GFP
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human DUSP5 promoter
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217418-plasmid-map-sequence-id-434921

    Enlarge

  38. AAV-sCAG-eGFP-ACAP1

    Plasmid
    #203800
    Purpose
    AAV vector plasmid expressing human ACAP1 fused to eGFP under a short variant of the CAG (sCAG) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva
    Insert
    ACAP1 (ACAP1 Human)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    203800-plasmid-map-sequence-id-410892

    Enlarge

  40. FLAG-Ex1-17-deltaP1P2-PTPN22

    Plasmid
    #195391
    Purpose
    Mammalian expression of a human PTPN22 mutant (exon 1-17 without the P1 and P2 domains) with FLAG tag
    Depositor
    Patrick Concannon
    Insert
    PTPN22 (PTPN22 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    PTPN22 mutant containing exon 1 to 17 and lacking…
    Promoter
    Available since
    April 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    195391-plasmid-map-sequence-id-389527

    Enlarge

  42. pAc5.1A-DmXRN1-P1341AP1342A-V5His6_Q

    Plasmid
    #147252
    Purpose
    Insect Expression of DmXRN1-P1341AP1342A
    Depositor
    Elisa Izaurralde
    Insert
    DmXRN1-P1341AP1342A (pcm Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    one silent mtuation compared to the sequence give…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147252-plasmid-map-sequence-id-289699

    Enlarge

  43. FLAG-Ex1-17-deltaP1-PTPN22

    Plasmid
    #195388
    Purpose
    Mammalian expression of a human PTPN22 mutant (exon 1-17 without the P1 domain) with FLAG tag
    Depositor
    Patrick Concannon
    Insert
    PTPN22 (PTPN22 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    PTPN22 mutant containing exon 1 to 17 and lacking…
    Promoter
    Available since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195388-plasmid-map-sequence-id-387198

    Enlarge

Showing: 401 - 450 of 2096 results