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Showing: 401 - 450 of 23406 results

  4. pCDNA5/FRT_CMV_NES-Kinprola_on-mEGFP

    Plasmid
    #233355
    Purpose
    CMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on in mammalian cell lines fused to mEGFP
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Insert
    NES-Kinprola_on-mEGFP
    Use
    Tags
    NES and mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233355-plasmid-map-sequence-id-466631

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  9. pLKO.1-puro_CMV_NES-Kinprola_on-mEGFP

    Plasmid
    #233370
    Purpose
    CMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Insert
    NES-Kinprola_on-mEGFP
    Use
    Lentiviral
    Tags
    NES and mEGFP
    Expression
    Mutation
    Promoter
    CMV
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233370-plasmid-map-sequence-id-466616

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  17. Linked Free NES (AG4TGGA)2

    Plasmid
    #182488
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence (AG4TGGA)2 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Insert
    FPPS-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182488-plasmid-map-sequence-id-360434

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  18. Coexpressed Free deadFPPS-NES

    Plasmid
    #182492
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    deadFPPS-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10 and GAL7
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182492-plasmid-map-sequence-id-360446

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  34. DsRed-CPI-17-T38A-NES

    Plasmid
    #100791
    Purpose
    Mammalian expression of Fluorescent CPI-17 neutral mutation cytosolic
    Depositor
    Joseph Gordon
    Insert
    PPP1R14A (PPP1R14A Human)
    Use
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    T38A
    Promoter
    CMV
    Available since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    100791-plasmid-map-sequence-id-193234

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  35. DsRed-CPI-17-T38E-NES

    Plasmid
    #100789
    Purpose
    Mammalian expression of Fluorescent CPI-17 phospho mimetic cytosolic
    Depositor
    Joseph Gordon
    Insert
    CPI-17-T38E-NES (PPP1R14A Human)
    Use
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    T38E
    Promoter
    CMV
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    100789-plasmid-map-sequence-id-193220

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  37. pcDNA3-PKAsub(T/A)-YPet-NES

    Plasmid
    #138205
    Purpose
    Encodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub(T/A)-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
    138205-plasmid-map-sequence-id-307407

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  38. pcDNA3-PKAsub(T/A)-RLuc8N-NES

    Plasmid
    #138212
    Purpose
    Encodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub(T/A)-RLuc8N-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
    138212-plasmid-map-sequence-id-307414

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  40. pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

    Plasmid
    #174081
    Purpose
    Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.
    Depositor
    Ryan Mehl
    Article
    Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-
    Insert
    NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
    Use
    Tags
    NES-Flag
    Expression
    Mammalian
    Mutation
    L270G, Y271L, N311G, C313A
    Promoter
    CMV
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174081-plasmid-map-sequence-id-345715

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Showing: 401 - 450 of 23406 results